HPLC法测定参茸益气助阳胶囊中盐酸小檗碱的含量

目的 建立测定参茸益气助阳胶囊中盐酸小檗碱含量的高效液相色谱法.方法 采用Zorbax SB-C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈-0.1%磷酸溶液(50∶50)为流动相,检测波长为265 nm.结果 盐酸小檗碱在10～200μg·mL-1范围内线性关系良好(r=0.999 7),平均回收率为97.58%,RSD为1.65%.结论 采用高效液相色谱法测定盐酸小檗碱的方法准确、可靠、简便,可作为参茸益气助阳胶囊的定量分析方法.

Determination of berberine hydrochloride in Shenrong Yiqi Zhuyang Capsule by HPLC method

Objective To establish an HPLC method for the determination of berberine hydrochloride in Shenrong Yiqi Zhuyang Capsule.Methods The determination was performed on a Zorbax SB-C18(4.6 mm×250 mm,5μm)column with acetonitrile-0.1% phosphoric acid solution(50∶50) as mobile phase.The detection wavelength was 265 nm.Results The method showed good linear relationship within the range of 10～200μg·mL-1 (r=0.999 7),the average recovery was 97.58%(RSD=1.65%).Conclusion The established method was accurate,reliable and simple,which can be used for the quantitative analysis of Shenrong Yiqi Zhuyang Capsule.