| 一种新的大鼠脑胶质瘤动物模型的建立 |
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| 引用本文： | Xing B,Ren Z,Su C. 一种新的大鼠脑胶质瘤动物模型的建立[J]. 中国医学科学院学报, 1998, 20(4): 241-245 |
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| 作者姓名： | Xing B Ren Z Su C |
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| 作者单位： | 中国医学科学院中国协和医科大学协和医院北京100730 |
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| 基金项目： | 高等学校博士学科点专项科研项目,94-70, |
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| 摘 要： | 目的 以ＬａｃＺ基因为报告基因,建立新的大鼠脑胶质瘤动物模型。方法 用ＡＢＧ质粒转染Ｃ６胶质瘤细胞,体外观察并比较了野生型和转基因瘤细胞的生长周期、细胞增殖情况和恶性侵袭能力,利用立体定向技术建立了大鼠颅内外的肿瘤实验动物模型。结果 基因转染瘤细胞和野生型瘤细胞的生长特性和致瘤能力无明显差别。结论 以转基因瘤细胞建立的动物模型,能从细胞水平观察到胶质瘤的侵袭范围,为进一步进行脑胶质瘤的放疗人化疗实
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| 关 键 词： | 胶质瘤 动物模型 立体定向技术 脑肿瘤 |
| 修稿时间： | 1997-09-15 |
A new rat brain-glioma model |
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| Xing B,Ren Z,Su C. A new rat brain-glioma model[J]. Acta Academiae Medicinae Sinicae, 1998, 20(4): 241-245 |
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| Authors: | Xing B Ren Z Su C |
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| Affiliation: | PUMC Hospital, CAMS and PUMC, Beijing 100730. |
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| Abstract: | OBJECTIVE: To build a new rat brain glioma model with LacZ as a reporter gene. METHODS: Plasmid BAG was transfected into C6 glioma cell. The cell cycle, cell proliferation, malignant infiltrative ability of wild and transfected glioma cell were observed. Rat glioma model was built using stereotaxic implantation. RESULTS: There was no difference between transfected and wild glioma cell in cell growth characteristics and tumorigenesis. CONCLUSIONS: It was easy to observe the infiltrative area on cellular level from the rat glioma model made by transfected tumor cell. The method is satisfactory for radiation and chemotherapeutic trials of glioma. |
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