| 抗PTD-bcr/abl单克隆抗体的制备及鉴定 |
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| 引用本文： | 陈任安,刘利,蒋姗姗,梁英民. 抗PTD-bcr/abl单克隆抗体的制备及鉴定[J]. 细胞与分子免疫学杂志, 2008, 24(5): 488-490 |
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| 作者姓名： | 陈任安 刘利 蒋姗姗 梁英民 |
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| 作者单位： | 第四军医大学唐都医院血液科,陕西,西安,710038 |
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| 基金项目： | 第四军医大学校科研和教改项目 |
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| 摘 要： | 目的:制备单克隆抗体(mAb)并初步用于PTD-bcr/abl融合蛋白的研究,为慢性粒细胞白血病的免疫治疗提供实验依据.方法:在大肠杆菌中表达PTD-bcr/abl融合蛋白,用所获得的蛋白免疫BALB/e小鼠,常规收集小鼠脾细胞与Sp2/O细胞融合,筛选杂交瘤细胞株的阳性克隆,并对其进行一系列鉴定.结果:(1)表达PTD-bcr/abl融合蛋白;(2)制备了抗PTD-ber/abl融合蛋白mAb;(3)用mAb以多种方法检测PTD-bcr/abl融合蛋白,证实此抗体效价高,特异性强.结论:此抗体可用于PTD-ber/abl融合蛋白的进一步研究.
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| 关 键 词： | PTD-ber/abl融合蛋白 单克隆抗体 制备 单克隆抗体 monoclonal antibodies characterization 抗体效价 检测 结果 阳性克隆 杂交瘤细胞株 筛选 细胞融合 小鼠脾细胞 BALB 蛋白免疫 表达 大肠杆菌 方法 实验 免疫治疗 慢性粒细胞白血病 研究 |
| 文章编号： | 1007-8738(2008)05-0488-03 |
| 修稿时间： | 2007-07-19 |
Preparation and characterization of the monoclonal antibodies to PTD-bcr/abl |
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| CHEN Ren-an,LIU Li,JIANG Shan-shan,LIANG Ying-min. Preparation and characterization of the monoclonal antibodies to PTD-bcr/abl[J]. Chinese journal of cellular and molecular immunology, 2008, 24(5): 488-490 |
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| Authors: | CHEN Ren-an LIU Li JIANG Shan-shan LIANG Ying-min |
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| Affiliation: | Department of Haematology of Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China. |
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| Abstract: | AIM: To Preparation and characterization the monoclonal antibody (mAb)to PTD-bcr/abl fusion protein. METHODS: The PTD-bcr/abl fusion protein was expressed in E.coli. BALB/c mice were immunized with specific PTD-bcr/abl. The splenic cells of the mice were fused with Sp2/0 mouse myeloma cells. The hybridoma culture supernatants were screened by indirect ELISA. The property of the antibody was identified by Western blot and immunohistochemistry. RESULTS: The PTD-bcr/abl fusion protein and its mAb were obtained. mAb against PTD-bcr/abl displayed strong specifility and high affinity. CONCLUSION: An anti-PTD-bcr/abl fusion protein mAb with high affinity was abtained, which could be applied to the research of PTD-bcr/abl. |
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