CTLA-4Ig对复发性单纯疱疹性角膜基质炎的抑制作用

目的：研究CTLA-4Ig对复发性单纯疱疹性角膜基质炎（HSK）的抑制作用。方法：采用复发性HSK的BALB/c鼠模型，经紫外线B光照射角膜诱导HSK复发；使用CTLA-4Ig作为负性调节因子，观察其对复发性HSK的影响。结果：注射CTLA-4Ig的小鼠，复发性HSK的角膜混浊程度及角膜炎性细胞浸润明显减轻，迟发型超敏反应明显减弱，外周血中CT4^ T细胞减少了92.8％。结论：CTLA-4Ig通过阻断B7:CD28/CTLA-4协同刺激途径，抑制了CD4^ T细胞的增殖，阻止了复发性HSK的发展。

Inhibition of CTLA-4Ig fusion protein on recurrence of herpetic stromal keratitis

Objective: Our purpose was to investigate the inhibition of T cell activation with the fusion protein CT-LA-4Ig on the recurrence of herpetic stromal keratitis (HSK). Methods: In a mouse model of recurrent HSK, latently infected BALB/c mice intraperitoneally received CTLA-4Ig or IgG Fc or PBS given on the 0,2,4 days after ultraviolet-B-stim-ulated reactivation of herpes simplex virus. Results: Severity of lesions in CTLA-4Ig-treated mice markedly reduced, which was judged by using both slit lamp biomicroscopy and histopathology. Treated animals also had 92. 8% of reduction in the numbers of CD4+T cells measured by using flow cytometry and minimal delayed-type hypersensitivity. Conclusion: Blockade of coreceptor interaction between T cells and antigen-presenting cells with CTLA-4Ig can significantly reduce the severity of recurrent HSK.