益肝冲剂质量标准研究

目的 建立苴肝冲剂的质量标准。方法 采用TLC法对制剂中延胡索和厚朴进行定性鉴别；采用HPLC法测定丹参酮ⅡA的含量。选择C18柱。以甲醇-水（75：25）为流动相，流速为1．2mL·min^-1。检测波长为270nm。结果 在TLC图谱中可检出延胡索和厚朴的特征斑点。丹参酮IIA在7．92μg·mL^-1～79．2μg·mL^-1的浓度范围内线性关系良好,r=0．9995，平均回收率为98．5％，RSD为1．91％（n=5）。结论 该方法简便,准确,专属性强，可用于该制剂的质量控制。

Quality standard study for Yigan powder

OBJECTIVE To establish the quality standard for Yigan powder. METHODS Rhizoma Corydalis and Cortex Magnoliae Officinalis in the medicine were identified by TLC, the Tanshinone Ⅱ A content in this medicine was determined by HPLC.The methanol-water（75 ： 25）solulion was used as the mobile phase, the column was C18, the flow rate was 1.2mL·min^-1, and the detective wavelength was at 270nm. RESULTS Rhizoma Corydalis and Cortex Magnliae Officinalis could be indentified by TLC. Tanshinone ⅡA showed a good linear relationship at the range of 7.92μg·mL^- 1 - 79.2μg·mL^-1, r = 0. 9995. The average recovery was 98.5 %, and RSD was 1.91% （n = 5）. CONCLUSION The method is simple, reliable, accurate and can be applied to the quality control of the preparation.