分泌性中耳炎猫中耳粘膜SP和NPY含量的变化

目的：探讨Ｐ物质（ＳＰ），神经肽Ｙ（ＮＰＹ）在分泌性中耳炎发病过程中的作用。方法：阻塞咽鼓管建立分泌性中耳炎模型。用免疫组化ＡＢＣ－ＧＤＮ及图像分析技术研究中耳粘膜ＳＰ，ＮＰＹ的分布及含量的变化。结果：中耳粘膜ＳＰ免疫反应（ＳＰ－ＩＲ）阳性纤维定位分布多与血管无关，ＮＰＹ免疫反应（ＮＰＹ－ＩＲ）阳性纤维多伴中小动脉走行。咽鼓管阻塞８ｈ后，中耳即有明确渗出，中耳粘膜ＳＰ－ＩＲ阳性纤维２４ｈ开始增粗、

The content changes of substance P and neuropeptide Y in cat middle ear mucosa in secretory otitis media

Objective: This experiment aimed to investigate the effects of substance P (SP) and neuropeptide Y (NPY) on the invasion of secretory otitis media. Methods: Obstructing eustachian tube was used to establish the model of secretory otitis media. Immunohistochemistry ABC GDN and image analysing technology were adopted to study the distribution and content changes of SP and NPY in middle ear mucosa. Results: The distribution of positive SP IR fibrae of SP immunoreaction in middle ear mucosa seemed not to be related to blood vessels. Most positive NPY IR fibrae were accompanied by medium or small arteries. Middle ear exudation was observed 8 h after obstruction. Positive SP IR fibrae appeared to increase 24 h after obstruction. To 72 h, positive SP IR fibrae increased obviously in quantity and in staining. In 7 day group, positive SP fibrae became thicker, but there quantities seemed to remain the same. There was positive correlation between absorbance changes of positive SP IR products and quantity of exudation in middle ear mucosa. Middle ear NPY IR was not affected by secretory otitis media. Conclusions: SP might be the important inflammatory medium of secretory otitis media. Middle ear effusion might not be related to NPY.