白细胞介素2对淋巴细胞特异性蛋白酪氨酸激酶在肾小管上皮细胞中表达的影响

目的：探讨IL-2对肾小管上皮细胞中淋巴细胞特异性蛋白酪氨酸激酶（Lck） mRNA及蛋白表达的影响以及Lck在狼疮肾炎中的致病作用.方法：采用BALB/c小鼠及BXSB狼疮小鼠肾小管上皮细胞为实验对象,分别给予 IL-2（100 U/ml）刺激.应用逆转录聚合酶链反应（RT-PCR）方法检测肾小管上皮细胞Lck mRNA的表达,免疫印迹法检测Lck蛋白的表达,比较IL-2刺激前后Lck mRNA及蛋白表达水平的变化.结果：正常BALB/c小鼠及BXSB狼疮小鼠肾小管上皮细胞中仅有微量Lck mRNA及蛋白表达,经IL-2刺激后二者的Lck mRNA及蛋白表达水平均有增加,以狼疮小鼠肾小管上皮细胞增加更为显著.结论：（1）肾小管上皮细胞中有Lck基因的表达.（2）肾小管上皮细胞经IL-2刺激后Lck mRNA及蛋白表达水平增加,提示IL-2可活化肾小管上皮细胞诱导Lck表达,进而可能通过一系列信号传导作用,发挥生物学作用.相对于BALB/c小鼠,狼疮小鼠肾小管上皮细胞在IL-2刺激后Lck表达更明显,提示狼疮小鼠肾小管上皮细胞可能更易激活.（3）由于Lck在狼疮肾炎小鼠肾小管上皮细胞中异常表达,推测Lck可能作为细胞因子或炎症因子的重要信号分子,促进狼疮肾炎的发生.

Effect of IL-2 on Lck expression in renal tubular epithelial cells

Objective:To investigate the effect of interlenkin-2(IL-2) on Lck(Lymphocyte-specific protein-tyrosine kinase) gene expression in renal epithelial cells and the role of Lck on pathological process of Systemic lupus erythematosus(SLE).Methods:Tubular epithelial cells(TEC) derived from BALB/c and idiopathic SLE BXSB mice was exposed to IL-2(100 U/ml),the expression of lck mRNA and protein levels were estimated by RT-PCR and immunoblotting respectively.The differences of Lck gene expression between before and after IL-2 stimulations were compared.Results:The levels of Lck mRNA and protein were very low in TEC before IL-2 stimulations and increased sharply after IL-2 stimulations,the increases in TEC of BXSB mice ever higher.Conclusion:IL-2 could induces Lck gene expression in TEC especially in TEC of BXSB mice.Lck services as a important signal transduction molecule in IL-2 signalling pathway and may play a role in lupus nephritis pathogenesis.