白香丹胶囊对愤怒情绪模型大鼠海马GABABR和KIR表达的影响

目的检测愤怒情绪模型大鼠海马γ-氨基丁酸B受体两个亚基GABABR1、GABABR2和内向整流型钾通道(KIR)表达的变化,初步探讨白香丹胶囊对肝气逆证的中枢干预机制。方法大鼠随机分为正常对照组、模型组、白香丹胶囊组(0.2 g·kg-1)、巴氯芬组(0.008 g·kg-1),采用"社会隔离结合居住入侵"方法制备愤怒情绪大鼠模型,药物组按相应剂量灌胃给药7 d,每日1次。通过糖水偏好实验、旷场实验和高架十字迷宫实验进行模型评价,用免疫荧光化学法检测各组大鼠海马GABABR1、GABABR2和KIR的表达。结果与正常对照组比较,模型组糖水偏好系数降低,旷场实验得分增高,高架十字迷宫实验进入开放臂次数百分数(OE%)和开放臂停留时间的百分数(OT%)降低,海马GABABR1、GABABR2和KIR表达水平降低P<0.05;与模型组比较,白香丹胶囊组和巴氯芬组旷场实验得分降低,OE%和OT%值升高,海马GABABR1、GABABR2和KIR表达水平升高(P<0.05)。结论大鼠愤怒情绪的产生可能与海马GABABR1、GABABR2和KIR表达降低有关,白香丹胶囊平肝理气的中枢机制可能与其激活海马GABABR及其G蛋白偶联的KIR有关。

Effect of Baixiangdan Capsule on Expression of GABABR and KIR in Hippocampus of Anger-out Rat Models

Objective To detect the expression of two subunits of gamma aminobutyric acid B receptor,GABABR1 andGABABR2,as well as inwardly rectifying K+ channel(KIR) in hippocampus of anger-out rat models,and to explore the intervention mechanism of Baixiangdan Capsule in teating the syndrome of adverse flow of liver-Qi.Methods The Wistar rats were divided into normal group,modle group,Baixiangdan Capsule group(ig 0.2 g·kg-1,qd,for 7 d) and baclofen group(ig 0.008 g·kg-1,qd,for 7 d).The anger-out emotion rat models were established with social isolation plus resident intruder stress.The model was evaluated by sucrose intake test,open field test and elevated plus-maze test.The expression of GABABR1,GABABR2 and KIR in hippocampus was detected by immunoflourescence assay.Results Compared with the normal group,the modle group had lower sucrose intake,higher total score of open field test,lower open arm entry percent(OE %) and open arm time percent(OT %) of elevated plus-maze test,and lower expression of GABABR1,GABABR2 and KIR in hippocampus(P<0.05).Compared with the model group,Baixiangdan capsule and baclofen decreased the total score of open field test,increased OE % and OT % of elevated plus-maze test,and promoted expression of GABABR1,GABABR2 and KIR in hippocampus(P<0.05).Conclusion The lower expression of GABABR1,GABABR2 and KIR in hippocampus may have close relationship with anger-out emotion.Increasing the expression of GABABR and the function of G protein-coupled KIR in hippocampus maybe one of the mechanism of Baixiangdan capsule in soothing liver and regulating Qi flow.