吉西他滨对舌鳞癌细胞增殖和端粒酶活性的影响

目的:了解吉西他滨对舌鳞癌Tca8113细胞增殖及端粒酶活性的影响。方法:采用噻唑蓝(MTT)和碘化丙锭(PI)染色法,检测细胞增殖抑制率和细胞周期变化;采用TRAPPCRELISA法检测细胞端粒酶活性,PAGE-银染法显示端粒酶电泳图像。数据采用SPSS10.0中独立样本t检验法或单因素方差分析法进行统计学检验。结果:吉西他滨能够显著抑制Tca8113细胞增殖,并具有时间和浓度依赖性,5.0μg/ml组72h增殖抑制率均值为72.2%,高于0μg/ml组(F=161.854,P<0.05)。细胞周期检测显示:吉西他滨作用72h后,S期细胞比例下降,5.0μg/ml组均值为18.1%,低于0μg/ml组38.5%(t=5.801,P<0.05)。TRAPPCRELISA法检测细胞端粒酶活性(A值)随吉西他滨作用浓度增加和时间延长而下降:72h,0和5.0μg/ml组A值分别为1.32±0.12、0.28±0.02(F=811.208,P<0.05)。结论:吉西他滨能够有效抑制Tca8113细胞增殖,可以降低细胞端粒酶活性。

The effect of gemcitabine on proliferation and telomerase activity of Tca8113 cell lines

PURPOSE: To investigate the effect of gemcitabine on the proliferation and telomerase activity of Tca8113 cell line cells. METHODS: MTT assay, flow cytometry were used to examine the effect of gemcitabine on cell proliferation and cell cycle respectively, telomerase activity was detected with telomeric repeat amplification protocol (TRAP) and polyacrylamide gel electrophoresis (PAGE). The data were analyzed by independent samples t test and one-way ANOVA of SPSS10.0 for windows. RESULTS: Cell proliferations were significantly inhibited in these cells after exposure to gemcitabine as well as cell cycles were arrested. Furthermore, the effect was closely associated with the concentration and time of gemcitabine. The average rate of cell proliferating inhibition in group 5.0 microg/ml at 72 h was 72.2%.The number of cells in S phase was decreased,the average rate of S phase in group 5.0 microg/ml at 72 h was 18.1%,which was lower than that in group 0 microg/ml(38.5%) (t=5.801,P<0.05).Longer exposure time and higher concentration to gemcitabine, much telomerase activity was inhibited. After 72h, the A values of group 0 and 5.0 microg/ml were 1.32+/-0.12, 0.28+/-0.02, respectively (F=811.208, P<0.05). CONCLUSIONS: Gemcitabine could significantly inhibit Tca8113 proliferation and decrease its telomerase activity. Gemcitabine may be applied in clinical therapy of oral cancer.