SHR鼠颈上交感神经节内NPY的差异表达研究

目的　通过对自发性高血压大鼠（SHR）颈上神经节形态学及其内神经肽酪氨酸（NPY）表达变化的研究，探讨NPY在高血压发生发展中的作用。 方法　随机选取成年SHR和WKY各20只，观测颈上交感神经节的位置、形状、大小及重量，采用Real-time PCR技术和免疫组织化学法，检测两组大鼠颈上神经节内NPY mRNA和蛋白的表达。 结果　与同周龄的WKY组大鼠相比较，SHR组大鼠血压明显升高（P<0.05）；Real-time PCR和免疫组化结果显示：SHR颈上神经节内NPY mRNA和蛋白水平均较WKY增加（P<0.05）。 结论　NPY在基因转录和蛋白表达等方面均较WKY上调，并参与高血压的形成。

Differential expression of NPY in superior cervical ganglion of SHR rat

Objective To explore the effects of neuropeptide Y(NPY) on the occurrence and development of hypertension by observing its expression differentiation in superior cervical ganglion of spontaneous hypertensive rat(SHR).Methods We randomly selected SHR(n=20) and WKY(n=20) and observed the location,the shape,the size and the weight of the superior cervical ganglion.Real-time PCR and immunohistochemical PV-9000 method were used to examine the mRNA and protein expression of NPY in superior cervical ganglion of SHR and WKY.Results:The blood pressure of SHR was obviously higher than that of the WKY group(P<0.05).The results of Real-time PCR and the immunohistochemistry showed that the mRNA and protein expression of NPY in superior cervical ganglion of SHR increased obviously than those in WKY(P<0.05).Conclusions NPY may participate in the occurrence of hypertension by some complex mechanisms.