| 利用DNA池和变性高效液相色谱分析bcr和 abl基因序列标签位点多态性 |
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| 引用本文： | 田红,刘道明,徐兵,郑维扬,周淑芸. 利用DNA池和变性高效液相色谱分析bcr和 abl基因序列标签位点多态性[J]. 中国实验血液学杂志, 2005, 13(3): 468-471 |
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| 作者姓名： | 田红 刘道明 徐兵 郑维扬 周淑芸 |
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| 作者单位： | 1. 南京军区福州总医院血液内科,福州,350025
2. 第一军医大学南方医院血液科,广州,510515 |
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| 摘 要： | 为了探讨bcr和abl基因的单核苷酸多态性(SNP)与慢性髓细胞性白血病(CML)的关系，利用DNA池(DNA pooling)结合变性高效液相色谱(dHPLC)技术对bcr和abl基因上的9个序列标签位点(sequence-tagged site，STS)进行序列变异的筛查分析，并通过测序对筛查结果进行验证。研究结果表明，在9个STS片段中检出了4个片段中的多态性位点和3个片段中与参考序列不一致的变异。结论：U07000片段中的SNP在慢性髓性细胞白血病病人和对照人群中的基因频率分布有显著差异。
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| 关 键 词： | 序列标签位点 单核苷酸多态性 变性高效液相色谱 DNA池 慢性髓细胞性白血病 |
| 文章编号： | 1009-2137(2005)03-0468-04 |
| 修稿时间： | 2004-07-19 |
Analysis of Sequence-tagged Site in bcr and abl genes by DNA Pooling and dHPLC |
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| TIAN Hong,LIU Dao-ming,XU Bing,ZHENG Wei-yang,ZHOU Shu-yun. Analysis of Sequence-tagged Site in bcr and abl genes by DNA Pooling and dHPLC[J]. Journal of experimental hematology, 2005, 13(3): 468-471 |
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| Authors: | TIAN Hong LIU Dao-ming XU Bing ZHENG Wei-yang ZHOU Shu-yun |
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| Affiliation: | Department of Hematology, Fuzhou General Hospital of Nanjing Military Area, Fuzhou 350025, China. tianh710@hotmail.com |
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| Abstract: | To investigate the relationship between the single nucleotide polymorphism (SNPs) of the bcr and abl gene and chronic myelogeous leukemia (CML), the 9 sequence-tagged sites (STS) in bcr and abl gene were screened by DNA pooling and denaturing high performance liquid chromatography (dHPLC), and the results were varified by sequencing. The results showed that the polymorphism sites were detected in 4 out of the 9 STS fragments and there were 3 bases different from the reference sequence found in 3 fragments. In conclusion, the novel SNP in U07000 fragment shows significantly different frequencies between CML and controled people. |
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| Keywords: | sequence-tagged site single nucleotide polymorphism denaturing high performance liquid chromatography DNA pooling chronic myelogenous leukemia |
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