YKL－40和sCD36在实验性牙周炎大鼠血清中的表达

目的：观察大鼠实验性牙周炎基础治疗前后血清中YKL－40和sCD36的表达，探讨牙周炎与动脉粥样硬化（AS）之间的可能发病机制。方法：选取36只2月龄健康雄性Wistar大鼠，随机等分为3组，每组12只。正常对照组（A组）不作任何处理，2个实验组（B组和C组）通过丝线结扎上颌左右第二磨牙牙颈部，并配合牙周炎食谱及接种牙龈卟啉单胞菌（Pg）的方式建立牙周炎模型。建模成功后B组进行基础治疗，C组不进行牙周基础治疗，半个月后腹主动脉采血并处死大鼠，用酶联免疫吸附法检测YKL－40和sCD36的表达。结果：牙周炎未治疗组分别与空白对照组、牙周炎基础治疗组相比，血清YKL－40、sCD36含量均显著增高（P＜0．05）。结论：血清YKL－40、sCD36的水平在牙周炎组明显增加，且基础治疗后显著降低，这提示牙周炎的发生、发展过程可能影响血管壁的炎症反应，从而在动脉粥样硬化的发生、发展中有重要意义，但其确切机制尚需进一步的实验证实。

Expression of YKL-40 and sCD36 in serum of experimental periodontitis in rats

Objective：To investigate the expression of serum YKL-40 and sCD 36 in periodontitis in Rat before and after treatment,to explore the possible pathogenesis between periodontitis and atherosclerosis（AS）. Method：36 healthy 2-month-old male Wistar rats were randomly divided into three groups（n=12）. The rats with no treatment served as the con-trols（Group A）.The second premolars of other 2 groups were ligated with silk,fed with a periodontitis recipe and inoculated with P. gingivalis. After the success of modeling,B group were treated for half a month,abdominal aortic blood and rats were killed,the expression of YKL-40 and sCD36 was measured by ELISA. Result：Untreated periodontitis group respec-tively with the control group,the treatment of periodontitis -based group,compared with serum YKL-40,sCD36 levels were significantly higher （P〈0.05）. Conclusion：The levels of serum YKL-40,sCD36 was significantly increased in periodontitis groups,and significantly reduced after basic treatment .It suggests that the incidence of periodontitis may affect the devel-opment of the vascular wall inflammation, resulting in atherosclerosis in the occurrence and development of great signifi-cance. But its exact mechanism is still needed further study.