Production,characterization and use of monoclonal antibodies to grapevine virus A |
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| Authors: | D Boscia E Aslouj V Elicio V Savino M A Castellano G P Martelli |
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| Institution: | (1) Dipartimento di Protezione delle Piante, Università degli Studi and Centro di Studio del C. N. R. sui Virus e le Virosi delle Colture Mediterranee, Bari, Italy;(2) Istituto Agronomico Mediterraneo, Valenzano, Italy |
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| Abstract: | Summary Four stable hybridoma cell lines secreting monoclonal antibodies to grapevine closterovirus A (GVA) were obtained by fusing spleen cells of immunized BALB/c mice with mouse myeloma cell line Sp 2/0-Ag 14. In ELISA all MAbs reacted with virus in leaf extracts fromNicotiana benthamiana, glass-house-, field-, or in vitro-grown grapevines, or with cortical shavings from mature grape canes. In IEM tests, only one of the MAbs (PA 3.F 5) decorated virus particles on the entire surface. This MAb was likely induced by a surface antigenic determinant, whereas the other three MAbs (PA 3.D 11, PA 3.C 6, and PA 3.B 9) were originated by cryptotopes. Coupling polyclonal antibodies for coating protein A-sensitized plates, and monoclonal antibody conjugates for antigen detection, gave highly efficient and reproducible results for identification of GVA in field-grown grapevines. |
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