Integrin αvβ1 promotes infection by human metapneumovirus |
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| Authors: | Gabriella Cseke Melissa S Maginnis Reagan G Cox Sharon J Tollefson Amy B Podsiad David W Wright Terence S Dermody and John V Williams |
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| Institution: | aDepartment of Chemistry, Vanderbilt University College of Arts and Sciences, Nashville, TN 37232; and ;Departments of bMicrobiology and Immunology and ;cPediatrics and ;dElizabeth B. Lamb Center for Pediatric Research, Vanderbilt University School of Medicine, Nashville, TN 37232 |
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| Abstract: | Human metapneumovirus (hMPV) is a recently described paramyxovirus that causes lower respiratory infections in children and adults worldwide. The hMPV fusion (F) protein is a membrane-anchored glycoprotein and major protective antigen. All hMPV F protein sequences determined to date contain an Arg-Gly-Asp (RGD) sequence, suggesting that F engages RGD-binding integrins to mediate cell entry. The divalent cation chelator EDTA, which disrupts heterodimeric integrin interactions, inhibits infectivity of hMPV but not the closely related respiratory syncytial virus (RSV), which lacks an RGD motif. Function-blocking antibodies specific for αvβ1 integrin inhibit infectivity of hMPV but not RSV. Transfection of nonpermissive cells with αv or β1 cDNAs confers hMPV infectivity, whereas reduction of αv and β1 integrin expression by siRNA inhibits hMPV infection. Recombinant hMPV F protein binds to cells, whereas Arg-Gly-Glu (RGE)-mutant F protein does not. These data suggest that αvβ1 integrin is a functional receptor for hMPV. |
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| Keywords: | receptor paramyxovirus fusion protein viral entry |
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