p53功能失活在食管鳞癌中的表达及意义

目的：建立一种评价肿瘤生物学特性的新方法--p53功能失活检测法，并探讨p53功能失活与食管鳞癌TNM分期(tumor,nodes,metastasis staging）和组织学分级的关系。方法：采用p53功能测定法对45例新鲜食管鳞癌组织和正常食管组织进行p53功能检测（p53基因突变检测作为对照），将检测结果与患者的TNM分开期和组织学分级进行统计学分析。结果：p53功能失活率为64%，明显高于p53基因突变率49%。p53功能失活和食管鳞癌的TNM分期有关，分期越高，p53功能失活率越高；p53功能失活和食管鳞癌的组织学分级有关，分级越高，p53功能失活率越高。结论：p53功能失活有望成为一种评价食管鳞癌生物学特性的新指标；p53功能失活与食管鳞癌的TNM分期和组织学分级有关。

Significance of Functional Inactivation of p53 in Esophageal Squamous Cell Carcinoma

Objective： The current study was designed to establish a new method to evaluate biological activity of carcinoma— functional status of p53, and investigate the relationship between functional inactivation of p53 and the TNM(tumor,nodes,metastasis) staging or histological classification of squamous cell carcinoma of esophagus. Methods： A total of 45 samples of fresh esophageal tissues of squamous cell carcinoma and normal esophageal tissues were examined for functional inactivation of p53 by detection of functional inactivation of p53 ( comparison with detection of p53 gene mutation ) . Then the analyses of detected results and the TNM stagings or the histological classifications of the carcinoma were statistically analyzed in SPSS. Results： The rate of functional inactivation of p53 (64％ ) seemed to be obviously higher than that of p53 gene mutation (49％ ) with a significant difference (P=0.046). There was a significant relationship between functional inactivation of p53 and the TNM staging of esophageal squamous cell carcinoma. Its rate tended to be increased with the advance of the TNM staging; there was a significant relationship between functional inactivation of p53 and the histological classification. With the advance of the histological classification, the rate of functional inactivation of p53 could have increased. Conclusions： The functional inactivation of p53 would be expected to be a new method to evaluate the biological activity of esophageal squamous cell carcinoma； there was a significant relationship between functional inactivation of p53 and the TNM staging or the histological classification.