Hippo信号通路核心元件MST1在急性白血病患者中的表达及意义

本研究旨在探讨Hippo信号通路核心元件MST1基因在急性白血病患者中的表达情况及其与白血病发生、发展的关系。采用实时定量PCR检测50例初治急性白血病患者、33例正常人、23例完全缓解缓解患者及12例难治/复发患者的MST1基因的表达情况,采用Western blot进一步验证其蛋白的表达水平,结合临床资料对患者的预后因素进行分析。结果表明,与正常人相比,急性白血病初治患者MST1基因的表达水平明显减低(P<0.05),完全缓解前后差异有统计学意义(P<0.05),难治/复发患者与初治患者MST1基因表达水平无明显差异(P>0.05),完全缓解患者与正常人MST1表达水平无明显差异(P>0.05)。结论:MST1基因的低表达与急性白血病的发病及预后相关。

Expression of Hippo signaling pathway core element MST1 in acute leukemia patients and its significance

This study was aimed to investigate the expression of Hippo signaling pathway core element MST1 gene in acute leukemia (AL) patients, and explore its relation with AL pathogenesis and prognosis. 50 newly diagnosed patients with AL, 33 normal people, 23 patients with AL in complete remission, 12 refractory or relapsed patients were tested for the expression of MST1 gene by real-time quantitative PCR, Western blot was used to further validate the level of MST1 protein expression. And combined with clinical data, prognostic factors of the patients were analyzed. The results indicated that compared with the normal people, the expression level of MST1 in newly diagnosed patients with AL significantly decreased (P < 0.05), but significantly increased in AL patients with complete remission (CR), the difference of expression was statistically significant before CR and after CR (P < 0.05). Compared with refractory or relapsed patients, the expression level of MST1 gene in newly diagnosed patients was not significantly different (P > 0.05). Besides, the expression level of MST1 between the patients with CR and the normal people was not significantly different (P > 0.05). It is concluded that the low expression of MST1 may be related with the pathogenesis and prognosis of AL.