| 破骨细胞相关因子在骨再建过程中表达的定量分析 |
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| 引用本文： | 常新,侯志明,柴田恭明,塚崎智雄,山口朗. 破骨细胞相关因子在骨再建过程中表达的定量分析[J]. 华西口腔医学杂志, 2006, 24(2): 164-165 |
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| 作者姓名： | 常新 侯志明 柴田恭明 塚崎智雄 山口朗 |
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| 作者单位： | 大连医科大学附属第二医院,口腔科,辽宁,大连,116023;中国医科大学口腔医院,正畸科,辽宁,沈阳,110002;长崎大学生物医学院发展和再建医学部,口腔病理和生物医学工程分野,日本,长崎,852-8588 |
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| 基金项目： | 辽宁省自然科学基金;辽宁省博士科研项目;日本文部科学省博士后基金 |
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| 摘 要： | 目的 研究肿瘤坏死因子-α（TNF-α）、核因子-?资B受体激动剂（RANKL）、抗酒石酸酸性磷酸酶（TRAP）在骨再建过程中的表达。方法 用8周龄雄性C57BL/6J小鼠建立骨折模型，用RT-PCR法对骨形成过程中TNF-α、RANKL和TRAP的表达进行时程分析。结果 骨折后TNF-α、RANKL、TRAP的表达均上调, 但峰值在不同的时间点出现。TNF-α在骨折后2 d达峰值, RANKL的峰值出现在骨折5 d后, 而TRAP的表达在骨折后10 d达到峰值。结论 骨的再建也有破骨细胞相关因子的参与, 它有赖于成骨和破骨的精确平衡。
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| 关 键 词： | 肿瘤坏死因子 核因子-κB受体激动剂 抗酒石酸酸性磷酸酶 实时定量聚合酶链反应 骨再建 |
| 文章编号： | 1000-1182（2006）02-0164-02 |
| 收稿时间： | 2005-07-05 |
| 修稿时间： | 2005-07-052005-11-13 |
Quantitative Study of Osteoclastic Related Factors in the Process of Bone Reconstruction |
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| CHANG Xin,HOU Zhi-ming,SIBATA Yasuaki,TSUKAZAKI Tomoo,YAMAGUCHI Akira. Quantitative Study of Osteoclastic Related Factors in the Process of Bone Reconstruction[J]. West China journal of stomatology, 2006, 24(2): 164-165 |
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| Authors: | CHANG Xin HOU Zhi-ming SIBATA Yasuaki TSUKAZAKI Tomoo YAMAGUCHI Akira |
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| Affiliation: | 1. Dept. of Oral Science, The Second Affiliated Hospital of Dalian Medical University, Dalian 116023, China; 2. Dept. of Orthodontics, Stomatological School of China Medical University, Shenyang 110002, China; 3. Division of Oral Pathology and Biomedical Engineering，Dept. of Developmental and Reconstructive Medicine, Biomedical Sciences of Nagasaki University Graduate School，Nagasaki 852-8588, Japan |
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| Abstract: | OBJECTIVE: To detect the expression of osteoclast related factors, tumor necrosis factor-alpha (TNF-alpha), receptor activator of NF-kappaB ligand (RANKL) and tartrate-resistant acid phosphatase (TRAP), in the process of bone remodeling. METHODS: 8-week-old male C57BL/6J mice were employed in this study to detect the expression of osteoclast related factors by real time PCR. RESULTS: TNF-alpha, RANKL and TRAP were up regulated in the process of bone remodeling, they reached the peak on day 2, 5 and 10 individually after injury. CONCLUSION: Osteoclast related factors also participate in bone remodeling, which depends on the delicate balance between bone formation and bone resorption. |
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| Keywords: | tumor necrosis factor, receptor activator of NF-kB ligand tartrate-resistant acid phosphatase real time polymerase chain reaction bone remodeling |
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