COX-2选择性抑制剂塞来昔布对裸鼠荷人子宫内膜腺癌的抑制作用

 目的 探讨环氧化酶-2（cyclooxygenase-2，COX-2）选择性抑制剂塞来昔布对人子宫内膜腺癌的治疗作用及其机制。 方法 建立人子宫内膜腺癌HEC-1B细胞裸鼠荷瘤模型，待成瘤后随机分为对照组与实验组，对照组予生理盐水口服2周，实验组分别予塞来昔布4mg/d和2mg/d口服2周。从治疗开始每3天计算瘤体积一次，描绘肿瘤生长曲线，治疗结束后处死裸鼠剥除瘤组织，计算抑瘤率，采用RT-PCR法测定移植瘤组织COX-2 mRNA的表达、免疫组化法测定COX-2蛋白的表达和微血管密度（MVD）。 结果 塞来昔布对裸鼠皮下移植瘤的生长具有明显的抑制作用，且随着药物浓度的增加，抑瘤作用逐渐增强（抑瘤率分别为 32.4％和48.6％），RT-PCR结果显示移植瘤组织COX-2 mRNA的表达逐渐减弱，免疫组化结果显示移植瘤组织 COX 2蛋白的表达及微血管密度（MVD）逐渐减少，实验组与对照组之间及各实验组之间的差异均有统计学意义（P <0.05），COX-2蛋白的表达与MVD数量呈正相关（r系数为0.921，P<0.01）。 结论 COX-2选择性抑制剂塞来昔布能够抑制裸鼠荷人子 宫内膜腺癌的生长，其机制可能与降低COX 2的表达、减少微血管的生成有关。

Inhibitory Effect of Cyclooxygenase-2 Selective Inhibitor Celecoxib on Growth of Human Endometrium Adenocarcinoma Xenografts in Nude Mice

Objective To investigate the therapeutical effect of cyclooxygenase-2 selective inhibitor Celecoxib on human endometrium adenocarcinoma and its mechanism.Methods Human endometrium adenocarcinoma xenografts were established in nude mice. When the diameter of tumor was about 5 mm,the mice were divided into three groups randomely,which were treated with normal sodium,celecoxib of low dose (4 mg/d) and celecoxib of high dose (2 mg/d) respectively for two weeks. The size of the tumors were calculated every 3 day...