全反式视黄酸促进胚胎神经干细胞诱导分化的研究

目的本研究观察全反式视黄酸（atRA）对于体外分离培养的胚胎神经干细胞（ENSCs）生长增殖和诱导分化的作用及其可能的分子机制。方法分离培养孕15dSD大鼠（E15 d SD Rattus）海马回ENSCs，采用不同组合成分的神经干细胞培养基培养ENSCs，利用MTF法检测其对于ENSCs存活和增殖的影响；采用不同组合成分的神经细胞诱导分化培养基培养ENSCs，利用细胞免疫荧光染色法鉴定ENSCs及atRA对于其诱导分化的影响。结果MTT实验结果表明，atRA^＋组、atRA^-组和DMSO组均出现ENSCs的增殖效果，而对照组则没有明显的增殖现象，其中atRA^-组最明显，DMSO组次之，atRA^＋组最弱。AtRA^＋组与atRA^-的ENSCs经过诱导分化后产生的神经细胞类型有较明显差异，并且atRA组处理产生的神经元是对照组的3倍左右。结论atRA能够抑制ENSCs的增殖，并且抵消神经生长因子对于ENSCs的促进有丝分裂作用，atRA还可以促进ENSCs定向诱导分化为神经元。

All-trans retinoic acid promoting the differentiation of embryonic neural stem cells

Aim To observe the promoting effects and mechanisms of all-trans retinoic acid(atRA)on the proliferation and differentiation of embryonic neural stem cells(ENSCs)which were derived in vitro.Methods The ENSCs were derived from the hippocampus of rattus embryos(E15d SD Rattus).The effects of different culture medium on growth and proliferation of ENSCs were determined by MTT assay.The characters of cell will be identified by immunofluorescence staining.The effects of atRA and different medium on differentiati...