Immune activation and toxicity evaluation of fresh Cordyceps militaris extracts by high-pressure processing |
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| Authors: | Shuang-Jie Zhu Jun-Jie Yang An Zhou |
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| Institution: | 1. Institute of Fresh Medicine, Hefei University of Technology, Hefei 230009, China;2. School of Biotechnology and Food Engineering, Chuzhou University, Chuzhou 239012, China;3. Institute of Fresh Medicine, Hefei University of Technology, Hefei 230009, China;4. China Department of Pharmaceutics, Anhui University of Chinese Medicine, Hefei 230038, China |
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| Abstract: | The immune activation and toxicity of fresh Cordyceps militaris extracts (HFCM) treated by high-pressure processing (HPP) were evaluated by spleen and thymus index, macrophages phagocytosis activity, lymphocyte proliferation, interleukin-2, interferon-γ level, and human kidney 293 cells, hepatic and kidney function, with the ultrasonic treatment as control (UFCM). The results showed the spleen and thymus index, macrophage function, lymphocyte proliferation, IL-2, and IFN-γ levels in HFCM groups were higher than that in UFCM groups by 11.45–12.43%, 11.52–13.95%, 8.92–11.05%, 8.98–9.23%, 9.3–12.66%, and 12.69–17.83%. HFCM was 21.97%, 21.5%, and 20.74% higher than UFCM in the contents of polysaccharide, cordycepin, and total flavonoids. HFCM showed no toxicity to the cell viability, kidney, and hepatic, while the cell viability in UFCM groups was decreased to 81.54%. So the HFCM was stronger than UFCM in immune activation with no toxicity, and can be used as fresh health food. |
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| Keywords: | Cordyceps militaris immune activation toxicity high-pressure processing mice |
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