Topical Quercetin and Resveratrol Protect the Ocular Surface in Experimental Dry Eye Disease |
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Authors: | Antonio Abengózar-Vela Chris S. Schaumburg Michael E. Stern Margarita Calonge Amalia Enríquez-de-Salamanca |
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Affiliation: | 1. IOBA, University of Valladolid, Valladolid, Spainhttps://orcid.org/0000-0002-9604-9946;2. Biological Sciences, Inflammation Research Program, Allergan, Inc., Irvine, CA, USA;3. IOBA, University of Valladolid, Valladolid, Spain;4. Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Valladolid, Spainhttps://orcid.org/0000-0001-8178-4836;5. Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Valladolid, Spainhttps://orcid.org/0000-0003-1274-7796 |
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Abstract: | Purpose: To determine the anti-inflammatory effect of quercetin (QCT), resveratrol (RES), and their combination in a dry eye disease (DED) model.Methods: 0.01% QCT, 0.1% RES, 0.01% QCT + 0.1% RES (QCT + RES) or vehicle were topically applied in a desiccating stress (DS) mice model. CD4+ T cells isolated from DS-exposed mice were transferred to athymic recipient mice. Corneal fluorescein staining, tear production, and tear cytokine levels were evaluated in DS-exposed mice, and conjunctival CD4+ T cell infiltration was evaluated in recipient mice.Results: QCT (p < 0.001) and QCT + RES (p < 0.05) reduced corneal staining in DS-exposed mice. IL-1α tear concentration was reduced by QCT, RES, and QCT + RES (p < 0.05, 0.01 and 0.01, respectively) compared to DS + vehicle mice. CD4+ T cells increased in recipients of DS-exposed mice (p < 0.05) and were lower in recipients of QCT- and RES-treated mice (p < 0.05).Conclusion: The anti-inflammatory effect of QCT, RES, and QCT + RES on DED-experimental model suggests that their topical application could be used for DED treatment. |
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Keywords: | Desiccating stress dry eye syndrome inflammation quercetin resveratrol |
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