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The Effect of K-ATP Channel Blockage During Erythropoietin Treatment in Renal Ischemia-Reperfusion Injury |
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| Authors: | Nuray Yazihan Haluk Ataoglu Guzin Ozelci Kavas Nalan Akyurek Burcu Yener Cengiz Aydm |
| Affiliation: | 1. Ankara University, Faculty of Medicine, Molecular Biology Research and Development Unite, and Pathophysiology Department, Ankara, Turkey;2. Ankara University, Faculty of Medicine, Molecular Biology Research and Development Unite, and Microbiology, Department, Ankara, Turkey;3. Ankara University, Faculty of Medicine, Pathophysiology Department, Ankara, Turkey;4. Gazi University, Faculty of Medicine, Pathology Department, Ankara, Turkey;5. Ankara University, Faculty of Medicine, Molecular Biology Research and Development Unite, Ankara, Turkey;6. Yuksek Ihtisas Education and Research Hospital, Clinic Biochemistry Laboratory, Ankara, Turkey |
| Abstract: | ATP dependent K channels (K-ATP) take part in the Erythropoietin (EPO) induced cardioprotection but these channel activations have role in cytoprotective role of EPO in the renal ischemia reperfusion (IR) damage is still unknown. For this purpose rats were pretreated with EPO (500 IU/kg) and/or K-ATP channel blocker glibenclamide (40mM/kg) i.p. before bilateral renal IR damage. Renal tissues were used for histological examination and measurement of caspase-3 and TNF-α levels. Renal functions were evaluated by glomerular filtration rate (GFR) fractional excretion of sodium (FENa) and potassium (FEK). Renal TNF-α and caspase-3 levels were decreased in both glibenclamide and EPO-treated IR rats compared to untreated rats. The protection afforded by the pretreatment with EPO alone was greater than that of administering glibenclamide alone. Application of glibenclamide at the same time partly abolished the cytoprotective effect of EPO treatment. K-ATP mediated cytoprotection is not the main mechanism of protective effect of EPO. |
| Keywords: | erythropoietin (EPO) renal ischemia reperfusion (IR) injury glibenclamide ATP dependent K channels glomerular filtration rate (GFR) fractional sodium excretion (FeNa) fractional potassium excretion (FeK) caspase-3 TNF-α |