Intraocular Detection of Herpes viruses by xTAG Liquid Chip Technology in Patients with Acute Retinal Necrosis |
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| Authors: | Chan Zhao Jie Yi Fangtian Dong Rongping Dai Hanyi Min Lin Zheng |
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| Affiliation: | 1. Ophthalmology Department, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, Chinameifen_zhang@hotmail.com;3. Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China;4. Ophthalmology Department, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China |
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| Abstract: | Purpose: To evaluate the performance of the xTAG liquid chip technology (xTAG-LCT) for etiological diagnosis of acute retinal necrosis (ARN).Methods: Fifteen vitreous and 3 aqueous samples from 18 ARN patients were analyzed by xTAG-LCT and multiplex PCR (mPCR)/quantitative PCR (qPCR).Results: xTAG-LCT revealed positive results in 17 of the 18 samples: 10 for Varicella Zoster Virus (VZV) alone; 5 for VZV and Epstein-Barr virus (EBV); 1 for herpes simplex viruses type 1 (HSV-1) and EBV; 1 for VZV, HSV-1 and EBV. While mPCR revealed the same results as xTAG-LCT for VZV and HSV-1 in all samples, only 2 of the 7 samples positive for EBV on xTAG-LCT were confirmed by qPCR. None of the 28 control vitreous samples from 8 non-ARN patients and 10 pair of cadaveric eyes was positive for any of the tested viruses.Conclusions: xTAG-LCT could be a useful alternative for etiological diagnosis of ARN. |
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| Keywords: | Acute retinal necrosis herpes viruses pathogen polymerase chain reaction xTAG liquid chip technology |
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