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Traditional Chinese drug ShuXueTong facilitates angiogenesis during wound healing following traumatic brain injury |
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| Authors: | Jin Xiaoying Shen Gang Gao Feng Zheng Xuesheng Xu Xiaoyan Shen Fang Li Gu Gong Jiangbiao Wen Liang Yang Xiaofeng Bie Xiaodong |
| Affiliation: | aDepartment of Traditional Chinese Medicine, Second Affiliated Hospital, School of Medicine, Zhejiang University, #88 Jiefang Road, Hangzhou 310009, China bDepartment of Neurosurgery, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China |
| Abstract: | ETHNOPHARMACOLOGICAL RELEVANCE: ShuXueTong injection is a traditional Chinese drug designed to treat the patients of "blood stasis and stagnation (yu xue yu zhi)", including subacute brain trauma. However, the mechanism of the therapeutic effect of ShuXueTong on traumatic brain injury is unknown yet. AIM OF THE STUDY: We hypothesized that ShuXueTong may promote the brain wound healing by facilitating angiogenesis. Thus this study was designed to explore this hypothesis. MATERIALS AND METHODS: By means of microvessel count, Western blotting, immunocytochemistry, methyl thiazolyl tetrazolium assay and etc., the effect of ShuXueTong on the angiogenesis of brain wound was studied and then its influence on the VEGF/VEGFR-2 pathway were explored. RESULTS: ShuXueTong facilitates angiogenesis in the brain wound and improves the neurological function of the traumatized rats. VEGF expression in the lesion was elevated due to ShuXueTong induction. The in vitro experiment revealed VEGFR-2 and SRF expression in the endothelial cells were enhanced when exposed to ShuXueTong for merely 1d. Moreover, ShuXueTong promoted the endothelial cell proliferation via the VEGF/VEGFR-2 pathway. CONCLUSIONS: The mechanism of the therapeutic effect of ShuXueTong on traumatic brain injury lies at least partly in the enhanced angiogenesis in the lesion. |
| Keywords: | ShuXueTong Angiogenesis Traumatic brain injury |
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