多不饱和脂肪酸对金黄地鼠血脂和肝LDL受体的影响

研究了多不饱和脂肪酸对金黄地鼠血脂和肝LDL受体的影响,以探讨多不饱和脂肪酸降低血清脂质的机制。将雄性金黄地鼠30只随机分为对照组和多不饱和脂肪酸组,前者喂基础饲料加0.12%胆固醇,后者再加喂20%的玉米油,5周后测血脂和肝脏LDL受体。结果表明,与对照组相比,多不饱和脂肪酸组血清TC,TG,LDL-C,VLDL-C均明显下降,而HDL-C则明显升高。体外实验发现,多不饱和脂肪酸组金黄地鼠肝匀浆与~(125)I-LDL特异结合位点显著高于对照组,而~(125)I-LDL与两组动物肝匀浆结合的亲和力无明显差别,提示多不饱和脂肪酸降低血脂与肝LDL受体增加密切相关。

THE EFFECT OF POLYUNSATURATED FATTY ACID ON SERUM LIPIDS AND HEPATIC LDL RECEPTOR IN GOLDEN HAMSTERS

The purpose was to study the effect of dietary polyunsaturated fats on serum lipids and LDL receptor,30 male Golden hamsters were divided into 2 groups,the control and the treatment group.The hamsters in the control group were fed with ordinary diets plus cholesterol(0.12%), the hamsters in the treatment group were fed with ordinary diets,cholesterol (0.12%)plus corn oil(20%).All animals were fed for 5 weeks, The results indicated as follows:the serum TC,TG,LDL-C,VLDL-C were decreased very significantly in treatment group(P<0.01),while HDL-C was increased significantly(P<0.05),kd of ~(125)I-LDL binding hepatic LDL receptor in two groups has no obvious difference,while the number of hepatic LDL receptor was increased significantly in the treatment group(P<0.01).The result of the present study suggests that lipid-lowering effect of polyunsaturated fatty acid is intimately associated with the eleva- tion of hepatic LDL receptors.