| 二硫化碳对小鼠心肌细胞肌浆网Ca2+-ATP酶基因表达的影响 |
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| 引用本文： | 罗兰,吴磊,陕光,谭晓东.二硫化碳对小鼠心肌细胞肌浆网Ca2+-ATP酶基因表达的影响[J].中国职业医学,2006,33(1):27-29. |
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| 作者姓名： | 罗兰 吴磊 陕光 谭晓东 |
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| 作者单位： | 武汉大学公共卫生学院卫生学教研室,湖北,武汉,430071 |
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| 基金项目： | 教育部优秀青年教师基金项目(Jyb200102) |
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| 摘 要： | 目的探讨二硫化碳(CS2)对小鼠心肌细胞肌浆网Ca2 -ATP酶(SERCA2 a)表达的影响机制。方法将48只昆明小鼠分为4组给予不同剂量CS2(0~800 mg/m3)吸入染毒5周后,提取心肌的总RNA,运用逆转录聚合酶链反应技术来检测染毒小鼠心肌细SERCA2 a表达水平的变化。结果染毒前后体重差异无显著性(P>0.05),随着染毒剂量的增大,小鼠心肌细胞SERCA2 a的表达逐渐降低,与对照组比较差异有显著性(P<0.05)。结论CS2能降低小鼠心肌SERCA2 a的mRNA表达水平。
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| 关 键 词： | 二硫化碳 心肌 Ca2 -ATP酶 |
| 文章编号： | 1000-6486（2006）01-0027-03 |
| 收稿时间： | 2005-08-09 |
| 修稿时间： | 2005-08-092005-09-15 |
Effect of CS2 on the expression of sarcoplasmic reticulum Ca2+-ATPase of cardiomyocyte in mice |
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| LUO Lan, Wu Lei, Shan Guang, et al.Effect of CS2 on the expression of sarcoplasmic reticulum Ca2+-ATPase of cardiomyocyte in mice[J].China Occupational Medicine,2006,33(1):27-29. |
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| Authors: | LUO Lan Wu Lei Shan Guang |
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| Institution: | School of Public Health, Wuhan University, Wuhan 430071 ,China |
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| Abstract: | Objective To explore the expression changes of cardiac muscle sarcoplasmic reticulum Ca~(2 )-ATPase in mice induced by CS_2.Methods 40 Kunming mice were divded into 4 groups and were exposed at the concentrations of 0~800 mg/m~3 CS_2 for 5 weeks respectively.The total RNA of the heart was extracted.Reverse translation polymerase chain reaction(RT-PCR) were used to examine the expression level of Ca~(2 )-ATPase.Results mRNA expression level of SERCA2a was decreased with CS_2 dosage increasing(P<0.05),when compared with the controls.Conclusions It was seemed that CS_2 could decrease mRNA expreesion of Ca~(2 )-ATPase. |
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| Keywords: | Carbon disulfide Cardiomyocyte Ca~(2 )-ATPaseP |
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