无水乙醇和硝酸银处理兔肠黏膜效果的对比研究

目的:以兔为模型,观察比较无水乙醇和硝酸银溶液去肠黏膜的效果,为黏膜处理的试剂选择提供思路。方法:将25只白兔随机分为五组,每组5只,取20cm回肠段做隔离肠襻。五组分别以无水乙醇8min(A)、无水乙醇15min(B)、6%硝酸银8min(C)、3%硝酸银15min(D)、生理盐水8min(E)处理隔离之肠段。4周末,用葡萄糖吸收试验测定隔离肠袢吸收功能并取标本观察病理学改变。结果:无水乙醇和硝酸银溶液均具有去肠黏膜作用,四个处理组较对照组在形态学方面具有明显萎缩改变(P<0.01),葡萄糖吸收试验提示处理后肠段吸收功能减弱(P<0.05)。无水乙醇8min和6%硝酸银8min处理肠黏膜的效果好,对黏膜的吸收和分泌功能均有较好的抑制作用,但两组间比较差异无统计学意义(P>0.05)。结论:无水乙醇灌注8min和6%硝酸银灌注8min处理兔肠黏膜效果确切,能有效抑制吸收和分泌功能。两种方法处理后,在病理形态上未见明显差别,硝酸银在处理黏膜时与乙醇相比无明显优势。

Comparative study of the de-epithelialization effects of intestine by dehydrated alcohol and by Silver Nitrate

Objective:To observe and compare the de-epithelialization effects of intestine treated by dehydrated alcohol and by Silver Nitrate in rabbit model. Methods:Twenty-five rabbits were randomly divided into five groups, five in each group. All rabbits were anesthetized before operation. A 20 cm ileum segment was isolated with intact vessel. The ileum segments were treated in each group respectively with:(A)dehydrated alcohol for 8 min,(B)dehydrated alcohol for 15 min,(C)6%Silver Nitrate solution for 8 min(D)3%Silver Nitrate solution for 15 min(E)0.9% Sodium Chloride for 8 min.At the end of four weeks, we observed the absorptive function of the segments through Glucose-absorption test and also observed the histological changes.Results:Both dehydrated alcohol and Silver Nitrate can effectively de_epithelialize intestinal segments. Comparing with the control group, the mucosa from four treatment groups were atrophied (significance<0.01).The Glucose-absorption test demonstrated the absorptive function of the ileum segments in treated groups was decreased(significance<0.05).The group A and group C has a better result, mucosa from the two groups has poorer absorptive and secretive function, but when comparing the histological changes between them, it shows no significant differences.Conclusions: Treatment of the ileum segment by dehydrated alchol for 8 min or by 6% Silver Nitrate solution for 8 min effectively depressed the absorptive and secretive function.