Stathmin-1作为子宫平滑肌肉瘤早期诊断及预后评估的分子标志物的研究

目的研究Stathmin-1在子宫平滑肌肿瘤中的表达及临床意义。方法选取2013年1月～2016年12月温州市人民医院及温州医科大学附属第一医院82例手术患者,分为典型子宫平滑肌肿瘤(UL组)30例、子宫富于细胞平滑肌肿瘤(CL组)30例、子宫平滑肌肉瘤(LMS组)22例,同时选择肌瘤旁正常肌层(M组)30例。采用免疫组化Envision二步法研究Stathmin-1在各组中的表达、临床病理学特征及预后的相关性。根据Stathmin-1表达水平,CL组及LMS组进一步分成低表达组及高表达组,进行复发及生存率分析。结果 Stathmin-1表达于细胞质,在子宫平滑肌肉瘤组的染色分值高于另三组(均P0.05)。Stathmin-1在子宫平滑肌肿瘤中的表达与患者年龄、肿瘤大小、肿瘤数目等临床病理学特征均无相关(均P0.05)。子宫富于细胞平滑肌中Stathmin-1高表达组较低表达组复发率高(P0.05)。子宫平滑肌肉瘤中Stathmin-1高表达组较低表达组生存率低(P0.05)。结论Stathmin-1在子宫平滑肌肉瘤中的高表达,提示检测Stathmin-1在早期辅助诊断子宫平滑肌肉瘤上有一定的临床意义。其在子宫平滑肌肿瘤中的表达水平与富于细胞平滑肌肿瘤的复发及子宫平滑肌肉瘤的预后可能相关。

Study on Stathmin-1 as a molecular marker for early diagnosis and prognosis evaluation of uterine leiomyosarcoma

Objective To study the expression and clinical significance of Stathmin-1 in uterine leiomyoma.Methods Eighty-two surgical patients of Wenzhou People''s Hospital and The First Affiliated Hospital of Wenzhou Medical University from January 2013 to December 2016 were enrolled and divided into the typical uterine leiomyoma group(UL group)with 30 patients,the uterus leiomyoma rich in cells group(CL group)with 30 patients and the uterine leiomyosarcoma group(LMS group)with 22 patients,and normal smooth muscular tissue of uterus(M group)with 30 patients at the same time.The Immunohistochemical Envision two-step method was used to study the correlation between the expression of Stathmin-1,clinicopathological features and prognosis in each group.According to the expression level of Stathmin-1,the CL group and the LMS group were further divided into a low expression group and a high expression group for recurrence and survival analysis.Results Stathmin-1 was expressed in the cytoplasm,and the staining score in the uterine leiomyosarcoma group was higher than that in the other three groups,respectively(P<0.05 for all).The expression of Stathmin-1 in the uterine leiomyoma was not correlated with the clinicopathological features such as patient age,tumor size and tumor number(P>0.05).In the uterus leiomyoma rich in cells group,the recurrence rate in the high Stathmin-1 expression group was higher than that in the low Stathin-1 expression group(P<0.05).The survival rate in the high Stathmin-1 expression group was lower than that in the low Stathmin-1 expression group(P<0.05).Conclusion The high expression of Stathmin-1 in uterine leiomyosarcoma suggests that the detection of Stathmin-1 has certain clinical significance in the early auxiliary diagnosis of uterine leiomyosarcoma.Its expression level in uterine leiomyoma may be associated with the recurrence of cell leiomyoma and the prognosis of uterine leiomyosarcoma.