持续灌注微泡并低强度超声介导体外血栓溶栓的实验研究

目的研究持续灌注微泡并低强度超声介导体外血栓的溶栓效果。方法构建体外循环系统,新鲜牛血栓随机分为5组。组1为低强度超声联合导管内持续灌注微泡和尿激酶进行溶栓;组2为低强度超声联合血栓内一次性注射微泡和尿激酶进行溶栓;组3为低强度超声联合导管内持续灌注尿激酶进行溶栓;组4为低强度超声联合导管内持续灌注微泡进行溶栓;对照组为导管内持续灌注尿激酶进行溶栓。统计分析各组的溶栓率和溶解面积,观察血栓内部红细胞分布情况及纤维蛋白网结构的完整性。结果组1溶栓率及溶栓面积均高于其他各组,差异均有统计学意义(P均<0.05);其他各组之间均无显著差异。该组HE染色镜下显示红细胞分布更松散,免疫荧光染色镜下可见较多纤维蛋白网结构断裂。结论在体外实验中,低强度超声联合持续灌注微泡能够增强尿激酶的溶栓效果。

Thrombolysis Mediatied By Continuous Perfusion of Microbubble and Low-intensity Ultrasound in Vitro

Objective To study the effect of thrombolysis mediatied by continuous perfusion of microbubbles and low-intensity ultrasound(LIUS)in vitro.Methods We constructed an in-vitro circulatory model.50 bovine blood clots were divided into 5 groups randomly.In group 1,LIUS combined with continuous perfusion of microbubbles and urokinase(UK)were conducted.In group 2,LIUS combined with one bolus injection of microbubbles and UK were conducted.In group 3,LIUS combined with continuous perfusion of UK were conducted.In group 4,LIUS combined with continuous perfusion of microbubbles were conducted.Group 5 was the control group in which only UK was continuously conducted.The thrombolysis rate and dissolved area of bovine blood clots of each group were calculated and analyzed statistically.The erythrocyte structure and the integrity of fibrin net were observed under the microscope.Results In group 1,the thrombolytic rate was higher than other groups and the dissolved area was larger than other groups,the difference was statistically significant(P<0.05).Examinations of HE stained sections showed that the distribution of erythrocyte in group 1 was sparser than other groups.More broken fibrin were observed under immunofluorescence microscope in group 1.There was no significant difference among the other groups.Conclusions In vitro,LIUS combined with microbubbles could enhance the thrombolytic effect of UK.