AGAP2-AS1对结肠癌细胞增殖、凋亡的影响

目的探究AGAP2-AS1在结肠癌组织中的表达及对结肠癌细胞增殖、凋亡的影响。方法从美国TCGA(The Cancer Genome Atlas)数据库结直肠数据下载结肠癌患者的RNA-Seq数据。通过TCGA数据库获得356例结直肠癌,145例健康样本,通过定量实时PCR(Polymerase Chain Reaction)检测结直肠癌细胞和正常肠黏膜上皮细胞AGAP2-AS1表达水平。用siRNA(Small interfering RNA)抑制结直肠癌细胞中AGAP2-AS1的表达,再次分析其细胞增殖、凋亡的改变。结果 AGAP2-AS1在结肠癌组织和细胞中表达均显著增加。抑制AGAP2-AS1后肿瘤细胞增殖能力显著降低,凋亡显著增加(P0.05)。结论 AGAP2-AS1在结肠癌细胞系中高表达,抑制AGAP2-AS1表达可抑制结肠癌细胞增殖,并诱导其凋亡。

Effects of AGAP2-AS1 on proliferation and apoptosis of colon cancer cells

Objective To investigate the expression of AGAP2-AS1 in colon cancer and its effect on the proliferation and apoptosis of colon cancer cells.Methods The colorectal cancer RNA-Seq data were downloaded from The Cancer Genome Atlas(TCGA) database.A total of 356 cases of colorectal cancer and 145 healthy samples were obtained from TCGA.The expression of AGAP2-AS1 in colorectal cancer cells and normal intestinal epithelial cells was detected by quantitative real-time polymerase chain reaction(PCR).Small interfering RNA(siRNA) was used to inhibit the expression of AGAP2-AS1 in colorectal cancer cells,and the changes of its proliferation and apoptosis were further analyzed.Results The expression of AGAP2-AS1 was significantly increased in colon cancer tissues and cells.After AGAP2-AS1 was inhibited,the proliferation of tumor cells was significantly reduced and apoptosis was significantly increased(P<0.05).Conclusion AGAP2-AS1 is highly expressed in colon cancer cell lines.Inhibition of AGAP2-AS1 expression can inhibit the proliferation of colon cancer cells and induce their apoptosis.