白藜芦醇对小细胞肺癌C-myc基因表达的调控研究

目的研究白藜芦醇对小细胞肺癌C-myc基因表达的影响，探讨其在小细胞肺癌中的可能抑癌机制。 方法以人小细胞肺癌H446细胞系为研究对象，采取Western blot法和Real-Time PCR法检测不同浓度白藜芦醇及白藜芦醇作用不同时间后C-myc的表达变化，分别以NAC和顺铂为干预进行验证。 结果Western blot和Real-time PCR检测结果显示白藜芦醇可调控人小细胞肺癌H446细胞C-myc的表达，并随着作用时间的延长和作用浓度的增加，C-myc的表达进一步下降，具有时间依赖和浓度依赖的特点，差异有统计学意义(P<0.05)。给予NAC干预后，可抑制白藜芦醇对C-myc蛋白表达的调控影响，呈现拮抗作用，差异均有统计学意义(P<0.05)。顺铂可抑制C-myc的表达，与白藜芦醇联用对C-myc调控具有协同作用，Western blot检测结果显示在胞浆蛋白中差异有统计学意义(P<0.05)，但在胞核蛋白中，与白藜芦醇组相比，白藜芦醇与顺铂联用，差异无统计学意义(P>0.05)。Real-time PCR检测结果显示顺铂可使C-myc表达水平下调，与白藜芦醇联用具有协同作用，差异有统计学意义(P<0.05)。 结论白藜芦醇对小细胞肺癌H446细胞的C-myc表达具有明显的影响，间接说明白藜芦醇对小细胞肺癌中的抑癌作用可能与myc基因有关。

Study of regulating effect of resveratoral on C-myc gene in human small cell lung cancer H446 cells

Objective The aim of the present study was to evaluate the effects of resveratrol on C-myc gene expression in small-cell lung cancer(SCLC)cell.Methods The H446 cell line of human small-cell lung cancer was used as the object of this study.The expression levels of C-myc were measured by western blot analysis and Real-time PCR in H446 cells treated with various concentrations of Res for 24 h and treated with the same concentration Res at different times.Cisplatin was used as the intervention for effect verification.Results The measure results of Western blot and Real-time PCR show that resveratrol has regulating effect on the expression of C-myc in H446 cells of human small-cell lung cancer.Along with the extension of the action time and the increase of the concentration,the expression of c-myc further decreased.These effects have the characteristic of concentration-and time-dependence,the difference has statistically significant(P<0.05).In addition,combined treatment with NAC and Res is found to be a inhibiting effect compared with either monotherapy while cisplatin and Res is found to be markedly more effective compared with either monotherapy,the difference has statistically significant(P<0.05).However,the result of Western blot shows that the expression of C-myc in the cytonuclear protein has no significant difference in the combination group and the resveratrol group(P>0.05).Conclusion The present study demonstrated that resveratrol has a significant effect on C-myc expression in H446 cells.These findings suggest that the inhibiting effect of resveratrol on small cell lung cancer may be related to myc gene.