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| 前列腺分泌蛋白94基因的克隆及序列测定 | |
| 引用本文： | 田长生,高瑞娟,李春凌,赵雪俭,刘树铮.前列腺分泌蛋白94基因的克隆及序列测定[J].中国病理生理杂志,2001,17(1):19-22. |
| 作者姓名： | 田长生 高瑞娟 李春凌 赵雪俭 刘树铮 |
| 作者单位： | 1. 白求恩医科大学病理生理教研室, 吉林 长春 130021; 2. 白求恩医科大学预防医学院, 吉林 长春 130021 |
| 基金项目： | 吉林省科技发展计划项目(No.20001110)； JICA(日本国际协力事业团)资助项目 |
| 摘 要： | 目的:作为构建前列腺分泌蛋白94(PSP94)分泌型表达质粒的基础。方法:从正常前列腺组织中钓取PSP94 cDNA,构建重组质粒pUC19－PSP94,转化后提取目的基因并进行测序。结果:(1)构建了pUC19－PSP94的重组克隆质粒;(2)PCR扩增获得了280bp的PSP94成熟蛋白基因;(3)测定了PSP94的基因序列。结论:所构建的pUC19－PSP94重组质粒可成功地克隆PSP94蛋白基因。 |
| 关 键 词： | 前列腺 分泌 蛋白质类 基因 质粒 |
| 文章编号： | 1000-4718(2001)01-0019-04 |
| 收稿时间： | 1999-10-08 |
| 修稿时间： | 1999年10月8日 |
Clone and sequence analysis of prostate secretory protein of 94 amino acids |
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| TIAN Chang-sheng ,GAO Rui-juan ,LI Chun-ling ,ZHAO Xue-jian ,LIU Shu-zheng.Clone and sequence analysis of prostate secretory protein of 94 amino acids[J].Chinese Journal of Pathophysiology,2001,17(1):19-22. | |
| Authors: | TIAN Chang-sheng GAO Rui-juan LI Chun-ling ZHAO Xue-jian LIU Shu-zheng |
| Institution: | 1. Department of Pathophysiology,Norman Bethune University of Medical Sciences, Changchun 130021, China; 2. Institute of Preventive Medicine, Norman Bethune University of Medical Sciences, Changchun 130021, China |
| Abstract: | AIM: To construct recombinant human prostate secretory protein of 94 amino acids(PSP94) expression vector. METHODS: The PSP94 cDNA was obtained from normal prostate tissue, and recombinant plasmid pUC19-PSP94 was constructed. The target gene was identified and sequenced. RESULTS: 1) The recombinant plasmid pUC19-PSP94 was constructed; 2) The mature PSP94 protein gene of 280bp was acquired by PCR; 3) The gene sequence of PSP94 was identified.CONCLUSION: The constructed plasmid pUC19-PSP94 could to be clone PSP94 successfully. |
| Keywords: | Prostate secretion Proteins Genes Plasmids |
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