脑缺血再灌注后不同时间点病理变化与细胞凋亡的研究

目的探讨脑缺血再灌注后不同时间点病理变化与细胞凋亡的特点。方法采用线栓法制作大鼠大脑中动脉阻断(MCAO)局灶性脑缺血再灌注模型,光镜下观察病理变化,TUNEL法检测细胞凋亡。结果1脑缺血2h再灌注1~6h皮质缺血区病理变化不明显,24~48h病理变化明显;2脑缺血2h再灌注1h皮质缺血区可见凋亡细胞,24~48h达高峰,72h开始下降。结论脑缺血2h再灌注1~6h皮质缺血区脑细胞凋亡较轻,24~48h明显加重,且与病理变化相对应。宜尽早采取有效的干预措施抑制细胞凋亡,减轻脑组织病理损害。

Study on the changes of pathology and cell apoptosis in different time point following cerebral ischemic reperfusion in rats

Objective :To study the changes of pathology and cell apoptosis in different timepoint following cerebral ischemic reperfusion in rats. Methods: Middle cerebral artery occlusion(MCAO) and the model of reperfusion following focal cerebral ischemia were made using an intraluminal filament method,the changes of pathology were observed by light microscope and the changes of cell apoptosis were detected using method of TUNEL.Results:① The change of pathology was relatively less from 1 hour to 6h, and was more from 24 to 48h in the cortex following cerebral ischemic reperfusion. ② Apoptotic positive cells were found in the cortex after 1h following 2h cerebral ischemic reperfusion, reached peak after 24~48h, and reduced after 72h. Conclusion :The secondary change of cell apoptosis is relatively less from 1 hours to 6h, and is more from 24 to 48h in the cortex following cerebral ischemic reperfusion. An effective intervention should be made as soon as possible to reduce cell apoptosis, and to reduce pathological injury