益气活血法对模拟高原低温环境下大鼠急性肺水肿的预防作用

目的观察和评价益气活血方预防模拟高原低氧环境大鼠急性肺水肿的效果。方法雄性SD大鼠80只,随机分成空白对照组、模型组、红景天组和益气活血组,各20只;除空白对照组和模型组给予生理盐水外,益气活血组和红景天组分别给予等效剂量相应中药煎剂,连续灌胃6 d;自灌胃4 d开始,除空白对照组常温常压环境饲养外,其余各组置于模拟高原低压氧舱中饲养。结果造模后,益气活血组精神活动尚可,未出现呼吸困难及冻伤,死亡较少,而模型组与红景天组冻伤及死亡率高;肺湿/干比值红景天组和益气活血组明显比模型组低(P0.01),红景天组、益气活血组与空白对照组之间及两中药组之间差异无统计学意义。双肺大体及组织学观察均可见益气活血组较模型组和红景天组有明显减轻。结论益气活血法预防高原肺水肿效果较好,本实验为"高原急性肺水肿病机关键是‘气虚血瘀’"的观点提供了有力支持。

Preventive Effect on Acute Pulmonary Edema under Simulated Plateau En- vironment in Rats Treated with the Therapy for Benefiting Qi and Activa- ting Blood

Objective To observe and evaluate the effect on acute pulmonary edema under simula- ted plateau environment treated with the formulas for benefiting qi and activating blood circulation in the rats. Methods Eighty male SD rats were randomized into a blank control group, a model group, a Rhodiola rosea group and a benefiting qi and activating blood circulation group ,20 rats in each one. The physical saline was given in the blank control group and the model group. The corresponding Chinese herbal decoction of the same dose was applied in Rhodiola rosea group and the benefiting qi and activating blood circulation group separately with gastric infusion continuously for 6 days. Since the 4th day of gastric infusion, except the feed- ing in common temperature and environment in the blank control group, the rats in the other groups were fed in simulated plateau low - pressure oxygen tank. Results After modeling, the rats were still of high energy and activity and had no dyspnea and frostbite and less rats were dead in the benefiting qi and activating blood circulation group. In the model group and Rhodiola rosea group, the morbidity of frostbite and death were high. In Rhodiola rosea group and the benefiting qi and activating blood circulation group,lung wet to dry ra- tio was lower apparently than that in the model group（P 〈0.01 ）. As compared with the blank control group and the comparison between Rhodiola rosea group and the benefiting qi and activating blood circulation group, the difference in lung wet to dry ratio was not significant. The gross and histology of lungs all indicated the obvious alleviation of the disease in the benefiting qi and activating blood circulation group as compared with the model group and Rhodiola rosea group. Conclusion The therapy for benefiting qi and activating blood circulation has good effect on prevention from altitude puhnonary edema. This experiment provides the powerful support for the thought that the key of altitude acute pulmonary edema is qi deficiency and blood stagnation in pathogenesis.