人乳头状瘤病毒在翼状胬肉发病机制中的作用

背景分子生物学研究表明，翼状胬肉为肿瘤型组织。国外一些地区的研究表明，翼状胬肉中均表达人乳头状瘤病毒（HPV），但国内尚未见类似报道。目的定量检测翼状胬肉中HPV的表达，探讨HPV在无锡地区翼状胬肉患者发病机制中的作用。方法收集无锡市第二人民医院眼科行翼状胬肉切除术的手术标本33例48眼，其中包括6例7眼复发性翼状胬肉标本和27例41眼初发性翼状胬肉标本，翼状胬肉组织研磨后提取上清液中HPV16／18DNA、HPV6／11DNA，用荧光定量聚合酶链反应（FQ—PCR）法定量检测标本中HPV的含量，以荧光扩增曲线表示。2例人宫颈癌组织和2例人正常供体结膜组织分别作为阳性对照组和阴性对照组。结果翼状胬肉、正常结膜和宫颈癌组织中的HPV6／11扩增曲线均位于临界阳性质控曲线之下；但宫颈癌组织中HPV16／18扩增曲线位于临界阳性质控曲线之上，而翼状胬肉组织和正常结膜组织扩增曲线处于临界阳性质控曲线之下。2例人宫颈癌组织中HPV16／18检测结果为阳性（2／2），HPV6／11检测结果为阴性（0／2）。翼状胬肉标本及正常结膜组织中HPV16／18、HPV6／11检测结果均为阴性（0／50）。结论HPV不是翼状胬肉发生和复发的必要条件，无锡地区翼状胬肉中的HPV感染率较低。

The role of human papillomavirus in the pathogenesis of pterygium

Background The pathogenesis of pterygium has been in the study.Relative molecular biology study showed that pterygium is a tumor-like lesion,and based on overseas literatures,human papillomavirus (HPV) is positively expressed in 100％ patients with pterygium in some region.However,if this result is suitable for Chinese patients is unclear.Objective This study was to identify the role of human HPV in the pathogenesis of pterygia in Wuxi area.Methods Forty-eight pterygium specimens including 7 recurrent pterygia and 41 primary pterygia were collected during the operation,and these patients were from Wuxi area.Two cervical carcinoma specimens and 2 conjunctiva specimens from normal donors were obtained as positive control and negative control respectively.The fluorescent quantitative polymerase chain reaction (FQ-PCR) was used to detect HPV DNA of specimens.Results The amplified curves of HPV 6,11 of pterygium specimens,cervical carcinoma specimens and normal specimens were all below the positive quality control curve,but the amplified curves of HPV16,18 were above the quality control curve in cervical carcinoma specimens; while those of pterygium specimens and normal conjunctival specimens were all below the quality control curve.HPV16/18 was identified in 2 cervical carcinoma specimens,but no HPV6/11 was detected in 2 cervical carcinoma specimens.However,HPV DNA expression in primary and recurrent pterygias were absent.Conclusions According to these results,HPV is not the primary cause for the pathogenesis of pterygium in Wuxi region.