去铁酮对糖尿病大鼠晶状体的抗氧化作用

背景氧化应激能促使糖尿病性白内障的发生,铁螯合剂可以减轻糖尿病患者的氧化应激反应。目的探讨去铁酮对糖尿病性白内障的干预作用。方法收集6周龄雄性Wistar大鼠40只,按随机数字表法分为4个组。正常对照组8只给予普通饲料喂养、腹腔内注射40mg／kg柠檬酸缓冲液,剩余32只大鼠采用高糖高脂饮食并腹腔内注射40mg／kg链脲佐菌素（STZ）法建立糖尿病模型,50mg去铁酮组、100mg去铁酮组分别给予50mg／kg和100mg／kg的去铁酮溶液灌胃,每日1次,共持续8周;糖尿病对照组造模后不做干预。于实验第8周用考马斯亮蓝测定各组大鼠晶状体中水溶性蛋白（WSP）、脲溶性蛋白（USP）和碱溶性蛋白（ASP）的质量浓度,分别用黄嘌呤氧化酶法、硫代巴比妥酸法和二硫代二硝基苯甲酸法测定晶状体匀浆中超氧化物歧化酶（SOD）活性以及丙二醛（MDA）含量和谷胱甘肽（GSH）的质量分数。结果各组大鼠晶状体中的WSP、USP和ASP质量浓度的差异均无统计学意义（F=1．73、0．18、0．09,P〉0．05）。50mg去铁酮组和100mg去铁酮组大鼠晶状体匀浆中MDA含量分别为（1．05±0．10）mmol／g、（1．05±0．22）mmol／g,均低于糖尿病对照组的（1．49±0．38）mmol／g,差异均有统计学意义（P〈0．05）。50mg去铁酮组和100mg去铁酮组大鼠晶状体匀浆中SOD活性和GSH质量分数分别为（321．29±16．57）U／mg、（322．07±22．16）U／mg和（7．83±0．65）mg／g、（7．70±0．77）mg／g,明显高于糖尿病组的（298．70±14．69）U／mg及（5．47±1．01）mg／g,差异均有统计学意义（P〈0．05）。50mg去铁酮组和100mg去铁酮组间上述各项指标间的差异均无统计学意义（P〉0．05）。结论去铁酮可以减轻晶状体的氧化应激反应,改善晶状体的能量代谢。

The anti-oxidative effect of deferiprone on ocular lens in diabetic rat

Background Oxidative stress is thought to be responsible to diabetes-complicated cataract.Our previous study demonstrated that as an iron chelator,deferiprone can protect lens from oxidative damage.Objective This further study aimed to investigate the role of deferiprone on the formation of diabetic-complicated cataract.Methods Forty 6-week-old Wistar rats were included in the study and randomized into 4 groups.Eight of them were used as the normal control group.Diabetes mellitus animal models were established in 22 rats by the carbonhydratediet and fat diet and the intraperitoneal injection of 40 mg/kg streptozocin (STZ).The deferiprone of 50 mg and 100 mg were intragastrically given in 8 model rats respectively after 3 days once a day for 8 weeks.The opacification of lenses was examined under the slit lamp weekly after treatment.The animals were sacrificed and the lenses were obtained at the eighth week of deferiprone injection.The concentrations of water-soluble protein ( WSP),urine-soluble protein (USP) and alkali-soluble protein (ASP) in rat lens suspension were detected by Bradford method.The super oxide dimutese (SOD),malondialdehyde (MDA) and glutathione (GSH) were determined spectrometically using xanthine oxidase,thiobarbituric acid,dithio bis-nitrobenzoic acid.Results No evidently differences were found in the content of the WSP,USP and ASP among the these groups( F=1.73,0.18,0.09,P＞0.05).The contents of MDA in 50 mg deferiprone group and 100 mg deferiprone group were ( 1.05 ± 0.10 ) mmol/g and ( 1.05 ± 0.22 ) mmol/g respectively,showing a significant decline in comparison with diabetic model group (P＜0.05).The SOD and GSH contents in lens were (321.29±16.57) U/mg,(322.07±22.16) U/mg and (7.83±0.65 ) mg/g,(7.70±0.77 ) mg/g respectively in 50 mg deferiprone group and 100 mg deferiprone group and were considerably elevated in comparison with ( 298.70± 14.69 ) U/mg and ( 5.47 ± 1.01 ) mg/g of diabetic model groups ( P＜0.05 ).No significant differences were found in the indexes mentioned above between 50 mg and 100 mg deferiprone groups(P＞0.05).Conclusions Deferiprone can reduce oxidative stress and improve the energy metabolism of the lens in diabetic rats.