基于离子液体复合膜的葡萄糖生物传感器

目的建立一种电化学葡萄糖生物传感器,进而评价其在血糖检测的应用前景。方法将葡萄糖氧化酶（GOD）分散在N,N-二甲基甲酰胺（DMF）-离子液体（[BMIM]PF6）复合膜中滴于二茂铁（Fc）修饰的丝网印刷碳电极（SPCE）上,制备一种新型三电极葡萄糖电化学生物传感器。用扫描电子显微镜（SEM）、循环伏安法（CV）和计时电流法表征生物传感器的制备以及其电化学特性。结果该生物传感器有良好的稳定性和灵敏性,响应电流与葡萄糖浓度在0.5～16mM之间有良好的线性相关,检出限为0.1mM。结论制备的电化学葡萄糖生物传感器具有良好性能,可用于血糖检测。

Glucose Biosensor Based on Ionic Liquid Composite Film

Objective To prepare a novel three electrodes electrochemical glucose biosensor, and its clinical application in serum glucose detection to be assessed. Methods Glucose oxidase (GOD) was entrapped in N, N-dimethylformamide (DMF)-ionic liquid ([BMIM] PF6) composite film and then the composite was coated on a ferrocene (Fc) modified screen printed carbon electrode to prepare a novel glucose biosensor. The charac- teristics of the biosensor were investigated by scanning electron microscopy (SEM), cyclic voltam...