法舒地尔对大鼠脑缺血再灌注后Rho激酶、Nogo-A表达的影响

目的观察法舒地尔对大鼠缺血再灌注后Rho激酶、Nogo-A表达的影响,探讨其可能的机制。方法将72只成年健康雄性SD大鼠随机分为3组:A假手术组、B缺血再灌注组、C法舒地尔治疗组,每组24只,再分为1 d、3 d、7 d、14 d 4个时间点,每个时间点6只。采用Zea-Longa线栓法建立大脑中动脉缺血再灌注(MCAO)模型,应用免疫组化法观察缺血侧海马CA1区Rho激酶、Nogo-A的表达。结果 (1)B组、C组各时间点Rho激酶表达均高于A组(P<0.05),术后7d最为明显,C组Rho各时间点表达较B组减少(P<0.05)。(2)B组、C组Nogo-A蛋白表达较A组明显增多(P<0.05),C组各时间点的表达较B组减少(P<0.05)。结论法舒地尔可有效抑制缺血再灌注后Rho激酶及Nogo-A的激活从而达到促使轴突再生的作用。

Effects of Fasudil on Rho kinase and Nogo-A expression in rats after cerebral ischemia-reperfusion

ObjectiveTo study the effects of Fasudil on Rho kinase and Nogo-A expression in cerebral ischemia-reperfusion rats,and explore the possible mechanisms.MethodsA total of 72 adult healthy male Spragne-Dawley(SD) rats were equally divided into sham operation group(group A),cerebral ischemia-reperfusion group(group B),Fasudil treatment group(group C).Thread embolism method was adopted to establish the middle cerebral artery ischemia-reperfusion(MCAO) model,the expressions of Rho kinase and Nogo-A in hippocampal CA1 area of rats were observed through the method of immunohistochemical technique.ResultsThe expressions of Rho kinase in group B and C which increase significantly at 7th day were higher than group A,the expression in group C was lower than that of group B.The expressions of Nogo-A in group B and C were significantly higher than those in group A at each time point,the expression in group C was lower than group B(P<0.05).ConclusionFasudil could inhibit the activation of Rho kinase and Nogo-A effectively after ischemia-reperfusion rats so as to achieve to promote axon regeneration.