腺苷预处理对大鼠缺血再灌注损伤的保护作用和机制

目的探讨腺苷预处理对脑缺血再灌注损伤的保护作用和机制。方法制作大鼠脑缺血再灌注损伤模型。将大鼠随机分为3组:假手术组(F组)、缺血再灌注组(IR组)、腺苷预处理组(AP组),用Zeal Longa 5级评分法进行神经功能评分;免疫组织化学法检测脑组织内碱性成纤维细胞因子(basic fibroblast growth factor,bFGF)的表达。结果 (1)神经功能评分AP组各亚组均小于IR组各亚组(P均<0.05),但大于F组各亚组(P均<0.05);(2)F组bFGF阳性表达极弱,IR组和AP组在脑缺血再灌注后2 h开始出现bFGF弱阳性表达并逐渐增强,24 h达高峰,72 h略有下降,6 h2、4 h、72 h时AP组bF-GF阳性表达较IR组相应各亚组增强(P<0.05)。结论 (1)腺苷预处理能减轻大鼠局灶性脑缺血再灌注损伤所引起的神经功能缺损症状;(2)腺苷预处理能增强中枢神经系统内bFGF的分泌,进而起到保护脑组织的作用。

Influence of adenosine preconditioning on bFGF of rats with local cerebral ischemia-reperfusion injury

ObjectiveTo study the protective mechanism of adenosine preconditioning on focal cerebral ischemia-reperfusion injury in rats.MethodsThe models of reperfusion were made.A total of 60 rats were randomly divided into three groups: sham operated group(F group),ischemia-reperfusion group(IR group) and adenosine preconditioning group(AP group).The nerves function of rats was evaluated by Zeal Longa score method and the expression of GFAP was detected by the means of immunohistochemical.Results(1)The nerves function scores of AP group were lower than those of each subgroup of IR group(P<0.05) and higher than those of F group(P<0.05).(2)The expression of bFGF masccline cells was less in F group.The expression of bFGF masccline cells began to increase in 2 h subgroup reached the peak in 24 h subgroup and weakened in 72 h subgroup in both IR and AP group.The amount of bFGF masccline cells in each subgroup of AP group were more than those in IR according groups(P<0.05).Conclusion(1)Adenosine preconditioning can reduce the neurologic impairment symptom caused by cerebral ischemia-reperfusion injury.(2)Adenosine preconditioning can significantly increase the expression of bFGF masccline cells in the central nervous system of rats after brain ischemia-reperfusion.