石榴皮活性成分治疗痤疮作用机制的网络药理学预测及实验研究＊

目的 采用网络药理学及实验验证的方法,探索皮类中药石榴皮治疗痤疮的作用机制。方法 利用网络药理学技术筛选石榴皮治疗痤疮的活性成分和作用靶点,采用String数据库对活性成分靶点与痤疮疾病靶点进行蛋白互作网络分析,并通过David数据库对其结果进行KEGG通路富集分析。基于以上结果,采用Cytoscape3.6.1软件构建石榴皮治疗痤疮的“成分-靶点-通路”网络关系图。通过动物实验,观察石榴皮多酚乳膏对金黄地鼠皮脂腺斑面积及PI3K蛋白表达情况的影响。结果 本研究共获得药效成分31个,药物靶点193个,痤疮靶点1371个,药效成分与疾病的交集靶点79个。石榴皮治疗痤疮的主要成分为槲皮素、山奈酚、木犀草素等;核心靶点为Akt1、IL-6、VEGFA和PTSG2;关键信号通路可能包括PI3K-Akt信号通路、TNF信号通路、T细胞受体信号通路、FoxO信号通路等。体内实验证实,石榴皮能够降低金黄地鼠皮脂腺斑PI3K蛋白表达水平,抗皮脂腺斑增生。结论 本研究预测了石榴皮治疗痤疮可能的作用机制,为“以皮治皮”理论在中医皮肤科的应用提供现代理论依据。

Mechanism of Pomegranate Peel Active Ingredient for Acne: A Network Pharmacology Prediction and Experimental Study

Objective To explore the mechanism of pomegranate peel in the treatment of acne based on the methods of network pharmacology and experimental verification.Methods The active ingredients and targets of pomegranate peel for acne treatment were screened using network pharmacology technology, and the protein interaction network analysis was performed between the active ingredient targets and acne disease targets via the String database, followed by KEGG pathway enrichment analysis via the David database. Based on the above results, Cytoscape 3.6.1 software was used to construct a "component-target-pathway" network of pomegranate peel for acne treatment. The effects of pomegranate peel polyphenol cream on sebaceous gland area and PI3K protein expression in golden rats were observed through animal experiments. Results In this study, a total of 31 pharmacodynamic components, 193 drug targets, 1371 target related to acne. The main components of Pomegranate peel in treating acne were quercetin, kaempferol, luteolin, etc. The key targets were Akt1, IL-6, VEGFA, PTSG2, etc. The key pathways might include signaling pathways of PI3K, TNF, T cell receptor, FoxO, etc. The vivo experiments have confirmed that pomegranate peel can reduce the protein expression level of PI3K in sebaceous gland spot of golden hamsters and anti-sebaceous gland spot hyperplasia. Conclusion This study predicted the possible mechanism of Pomegranate peel in the treatment of acne, and provided a modern theoretical basis for the application of the theory of "treating dermatosis with herbal cortex" in the dermatology of traditional Chinese medicine.