| 大鼠肝微粒体温孵体系中（＋）,（—）—黄皮酰胺及其代谢产物的LC—MS分析 |
| |
| 引用本文： | 姚庆强,杨树民.大鼠肝微粒体温孵体系中（＋）,（—）—黄皮酰胺及其代谢产物的LC—MS分析[J].药物分析杂志,2000,20(1):3-7. |
| |
| 作者姓名： | 姚庆强 杨树民 |
| |
| 作者单位： | [1]中国医学科学院 [2]中国协和医科大学药物研究所 |
| |
| 摘 要： | 目的：建立黄皮酰胺及其代谢产物的ＨＰＬＣ－ＥＳＩ－ＭＳ在线检测分析方法，并对未分离得到的微量代谢产物进行分析确证，探索ＬＣ－ＭＳ在代谢转化研究中的应用。方法：利用柱后补偿技术，采用正离子检测对大鼠肝微体中（＋），（－）－黄皮酰胺及其代谢产物进行ＨＰＬＣ－ＥＳＩ－ＭＳ分析，根据ＭＳ的碎片信息检测主要的代谢产物，特别是对未分离得到的代谢产物的结构碎片进行分析，确定其结构。结果：除检测出主要已知代谢产物
|
| 关 键 词： | 黄皮酰胺 肝微粒体 体外代谢 电喷雾 HPLC－ESI |
LC-MS Analysis of ( ) and (-)-Clausenamide and Their Metabolites in Rat Liver Microsomes |
| |
| Yao Qingqiang,Wang Yan and Wang Muzou.LC-MS Analysis of ( ) and (-)-Clausenamide and Their Metabolites in Rat Liver Microsomes[J].Chinese Journal of Pharmaceutical Analysis,2000,20(1):3-7. |
| |
| Authors: | Yao Qingqiang Wang Yan and Wang Muzou |
| |
| Abstract: | Objectives:The HPLC-ESI-MS on line analytical method was developed for the determination of the minute metabolites of ( ) and (-)-clausenamide after incubation with rat liver microsomes. Methods:The HPLC-MS analysis of ( ) and (-)-clausenamide and their metabolites was used with post-column modification and positive polarity.The structures of the metabolites were analyzed by their MS data. Results:Besides the main known metabolites,an unknown metabolite,3-dehydroclausenamide was determined. Conclusion:The metabolic pathway of clausenamide was complemented by this research and the LC-MS method is a very useful method in the research of drug metabolism. |
| |
| Keywords: | ( )and (-)-clausenamide liver microsome metabolism in vitro high performance liquid chromatography-mass spectrometry electrospray ionization |
| 本文献已被 CNKI 维普 等数据库收录！ |
