荧光定量PCR检测母脐血HBV-DNA与母婴传播的临床研究

目的探讨孕妇乙肝病毒含量与宫内感染的相关性及母乳喂养的临床指导。方法对我院分娩的120例HBV感染产妇,采用荧光定量PCR检测法分别检测其孕晚期的血清,产时的脐血及产后乳汁中HBV-DNA含量,同时采用酶联免疫法行脐血乙肝病毒标志物检测。结果脐血HBV-DNA含量与孕母血清HBV-DNA含量有相关性;乳汁中HBV-DNA含量与其血清中HBV-DNA含量相关,同时与血清HBeAg存在显著相关。结论宫内感染的发生率与孕妇血清HBV-DNA含量相关,孕晚期高病毒血症(>108cp/ml)与宫内感染显著相关;产妇血清高HBV-DNA含量,乳汁HBV-DNA异常(>103cp/ml)或HBeAg阳性者不适宜母乳喂养。

Association between cord blood HBV-DNA and HBV vertical transmission

Objective:To explore association between serous HBV level in pregnant woman and incidence of intrauterine HBV infection of infant and to provide the guidance for breast feeding.Methods:Serum of advanced stage pregnant woman, cord blood at childbirth and postpartum milk were examined by fluorescent quantitation PCR for HBV level in 120 pregnant women. The marker HBV-DNA of HBV infection was detected by enzyme linked immunosorbent assay in the cord blood.Results:Cord blood HBV level was correlated to serous HBV-DNA content. The breast milk HBV-DNA was correlated to serous HBV-DNA and highly correlated to HBeAg..Conclusion:Incidence of intrauterine HBV infection is correlated to serous HBV-DNA level and highly correlated to viremia in advanced stage pregnant (>108 cp/ml). It is recommended that breast feeding be not suggested in women with high serous HBV-DNA level, increased HBV-DNA content in the milk (>103 cp/ml) or positive HBeAg.