Biochemical characterization of 1,25(OH)2D3 receptors in chick embryonal duodenal cytosol |
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| Authors: | Y. Seino K. Yamaoka M. Ishida H. Yabuuchi M. Ichikawa H. Ishige H. Yoshino L. V. Avioli |
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| Affiliation: | (1) Department of Pediatrics, Osaka University School of Medicine, Osaka, Japan;(2) Yamasa Shoyu Company, Chiba, Japan;(3) Division of Bone and Mineral Metabolism, The Jewish Hospital of St. Louis, 63110 St. Louis, Missouri, USA |
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| Abstract: | Summary This study presents measurements of serum vitamin D metabolites, calcium and phosphorus as well as measurements of the equilibrium dissociation constant for duodenal 1,25(OH)2D3 receptor in 15-, 18-, 19-, and 20-day chick embryos in comparison to that in 1- and 118-day-old chicks and to vitamin D-deficient chicks. The present results showed that: (a) serum 1,25(OH)2D and 24,25(OH)2D levels rise from 15 and 18 to days 19 and 20 of embryonic development while serum phosphate levels are stable; (b) serum calcium levels rise at hatching to adult levels; (c) the duodenal 1,25(OH)2D3 receptor is detectable in 15-day-old embryo and has a Kd similar to that of 118-day-old vitamin D-replete chicks; and (d) the activity of 1,25(OH)2D3 receptor in chick duodenal cytosol is maximal at hatching. |
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| Keywords: | 1,25(OH)2D3 Receptor Chicken Duodenal Cytosol Chicken Embryo Affinity 1,25(OH)2D3 Concentration |
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