| 用转染m2胆碱受体基因的CHO细胞观察知母皂甙元的作用 |
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| 引用本文： | 施菊,胡雅儿,易宁育,夏宗勤.用转染m2胆碱受体基因的CHO细胞观察知母皂甙元的作用[J].上海交通大学学报(医学版),2000,20(6):503-505. |
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| 作者姓名： | 施菊 胡雅儿 易宁育 夏宗勤 |
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| 作者单位： | 上海第二医科大学细胞调控研究室,上海 |
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| 基金项目： | 国家自然科学基金资助项目(39570835) |
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| 摘 要： | 目的 观察知母甾体皂甙元（ｓａｒｓａｓａｐｏｇｅｎｉｎ，ＳＡＲ）对Ｍ２受体亚型的调节作用。方法 采用转染了ｍ２胆碱受体基因的ＣＨＯ细胞为模型，以非选择性的＾３Ｈ－ＱＮＢ作点放射配基结合分析。结果 ＣＨＯｍ２细胞受体密度在培养４８ｈ达顶峰，以后呈进行性下降，ＳＡＲ对下降的ＣＨＯｍ２细胞Ｍ２受体有有显的上调作用，这一作用有一定浓度依赖性。结论 ＳＡＲ对下降的Ｍ２受体密度具有上调作用。
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| 关 键 词： | M2受体亚型 CHOm2细胞 知母皂甙元 放射配基分析 |
| 文章编号： | 0258-5898(2000)06-0503-03 |
| 修稿时间： | 2000年1月1日 |
The Regulation Effect of Sarsasapogenin on M2 Receptor Density in CHO m2 Cell |
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| SHI Ju,HU Yaer,YI Ningyu,et al.The Regulation Effect of Sarsasapogenin on M2 Receptor Density in CHO m2 Cell[J].Journal of Shanghai Jiaotong University:Medical Science,2000,20(6):503-505. |
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| Authors: | SHI Ju HU Yaer YI Ningyu |
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| Abstract: | Objective To explore the regulation effect of sarsasapogenin on M 2 receptor density in CHO m2 cell. Methods The decreased muscarinic receptor density CHO m2 cell line was used as a model. The M 2 muscarinic receptor density was measured by 3H-QNB single point radioligand binding assay. Results The time course of M 2 cholinoceptor density of CHO m2 cell showed that the M 2 receptor density reached a peak at 48h after cultured and then dropped gradually. Sarsasapogenin could significantly up-regulate the M 2 receptor density. Conclusion Sarsasapogenin can up-regulate the M 2 receptor density. |
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| Keywords: | M 2 muscarinic acetycholine receptor m2 receptor gene transfected Chinese hamster ovary cells radioligand binding assay sarsasapogenin |
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