抗核抗体检测受试者核谱及核型特征分析

目的探讨不同人群抗核抗体(ANA)阳性检出率、核型、ANA谱阳性分布特征及其临床应用价值。方法收集331名受试者的ANA实验室结果，分析其阳性率、核型及ANA谱阳性分布特征。结果ANA阳性和高滴度检出率女性高于男性，检出率高峰出现在40~60岁人群。不同ANA核型阳性检出率女性均高于男性，不同年龄段的受试者核型以核颗粒型和胞浆颗粒型为主。男性除抗硬皮病70和抗史密斯阳性检出率略高于女性外，女性其他ANA谱阳性检出率均高于男性；女性ANA谱阳性检出率前三位为:抗SS A、抗着丝粒蛋白B和抗双链DNA抗体/抗SS B；不同年龄段ANA谱阳性抗体略有不同，主要以抗SS A和抗史密斯为主。ANA在自身免疫性疾病强直性脊柱炎、类风湿性关节炎、系统性红斑狼疮、干燥综合征中检出率分别为36.0%、60.3%、91.7%、100.0%，在感染和健康人群中为29.4%和14.6%；ANA谱方面，强直性脊柱炎、类风湿性关节炎、感染和健康人群未见特异性抗体；抗SS A、抗双链DNA抗体和抗核小体在系统性红斑狼疮中有较高阳性检出率，抗核糖核蛋白/抗史密斯、抗SS A、抗SS B和抗PM-Scl在干燥综合征中有较高阳性检出率。结论ANA核型及抗体谱在不同性别、年龄和疾病中有不同的表现，其中感染和健康人群有较高的阳性率，提示临床鉴别诊断时给予重视。

Analysis of the nuclear spectrum and karyotype charaeteristics of subjects with antinuclear antibody

ObjectiveTo investigate the positive detection rate, karyotype and distribution characteristics of antinuclear antibody(ANA) positive spectrum of ANA in different populations.MethodsThe laboratory results of ANA in 331 subjects were collected, and the positive rate, karyotype and distribution characteristics of ANA positive spectrum were analyzed.ResultsThe detection rate of ANA-positive and high-titer in female was higher than that in male, and the highest detection rate appeared in people aged 40-60 years.The positive detection rate of different ANA karyotypes in female was higher than that in male.The karyotypes of subjects at different ages were main nuclear granule type and cytoplasmic granule type.The positive rates of anti-scleroderma 70 and anti-Smith in male were slightly higher than those in female, and the positive rates of other ANA spectra in female were higher than those in male.The top three positive rates of ANA spectrum in female were as follows: anti-SS A, anti-centromeric protein B and anti-dsDNA antibody/anti-SS B.The positive antibodies of ANA spectrum in different age groups were slightly different, which mainly concluded anti-SS A and anti-Smith.The detection rates of ANA in autoimmune diseases ankylosing spondylitis, rheumatoid arthritis, systemic lupus erythematosus and Sjogren′s syndrome were 36.0%, 60.3%, 91.7% and 100.0%, respectively, while the detection rates of ANA among infected and healthy people were 29.4% and 14.6%.As for ANA spectrum, no specific antibodies were found in ankylosing spondylitis, rheumatoid arthritis, infected and healthy people.Anti-SS A, anti-dsDNA antibody and anti-nucleosomes showed high positive detection rates in systemic lupus erythematosus, and anti-ribonucleoprotein/anti-Smith, anti-SS A, anti-SS B and anti-PM-Scl had high positive detection rates in Sjogren′s syndrome.ConclusionsANA karyotype and antibody spectrum have different manifestations in populations with different gender, age and diseases, among which infected and healthy people have a higher positive rate, which suggests that attention should be paid to in clinical differential diagnosis.