miR-18b-5p调控WWOX对胶质瘤细胞增殖、凋亡的影响

目的研究miR-18b-5p对胶质瘤U251细胞增殖、凋亡的影响和潜在的分子机制。方法qRT-PCR检测癌旁组织和胶质瘤组织中miR-18b-5p的表达，MTT法和流式细胞术检测胶质瘤U251细胞增殖和凋亡率，Western blotting检测包含WW域的氧化还原酶基因（WWOX）、Cyclin D1、p21、Bax和Bcl-2蛋白表达，双荧光素酶报告系统验证miR-18b-5p和WWOX的调控关系。结果与癌旁组织相比，胶质瘤组织中miR-18b-5p的表达量升高（P < 0.01）。抑制miR-18b-5p表达可以抑制胶质瘤U251细胞增殖并促进细胞凋亡；miR-18b-5p靶向负调控WWOX的表达；过表达WWOX可抑制U251细胞增殖并诱导细胞凋亡；抑制WWOX表达逆转了抑制miR-18b-5p表达对U251细胞增殖、凋亡的影响（P < 0.01）。结论miR-18b-5p可能通过靶向调控WWOX表达抑制胶质瘤U251细胞增殖并诱导细胞凋亡，miR-18b-5p是胶质瘤潜在的分子靶点。

miR-18b-5p regulates proliferation and apoptosis of glioma cells by targeting WW domam-containing oxidoreductase

ObjectiveTo investigate the effects of miR-18b-5p on the proliferation and apoptosis of glioma U251 cells and the underlying mechanism.MethodsThe expression levels of miR-18b-5p in glioma tissues and adjacent tissues were measured by quantitative real-time polymerase chain reaction(qRT-PCR). The proliferation and apoptotic rate of U251 cells were determined by MTT assay and flow cytometry. The expressions of WWOX, Cyclin D1, p21, Bax and Bcl-2 proteins were detected by Western blotting. The relationship between miR-18b-5p and WWOX was verified by dual-luciferase reporter assay system.ResultsCompared with the adjacent tissues, the expression of miR-18b-5p in glioma tissues was significantly increased(P < 0.01). Inhibition of miR-18b-5p inhibited the proliferation and induced apoptosis of U251 cells. MiR-18b-5p targeted and negatively regulated the expression of WWOX. Over-expression of WWOX inhibited the proliferation and induced apoptosis of U251 cells. Inhibition of WWOX reversed the effects of miR-18b-5p inhibition on cell proliferation and apoptosis of U251 cells(P < 0.01).ConclusionsMiR-18b-5p may inhibit the proliferation and induce apoptosis of glioma U251 cells by targeting WWOX. MiR-18b-5p is a potential molecular target of glioma.