胃癌患者外周血中CD4+PD-1+T细胞表型、功能变化及临床分期相关性研究

目的探讨CD4⁺PD-1⁺T细胞在胃癌患者外周血中的表型、功能及其与临床分期的相关性。 方法将江苏省常熟市医学检验所2019年1月至2020年12月收治的70例胃癌患者作为观察组，另选取50例健康体检者作为对照组，比较两组研究对象CD4⁺PD-1⁺T细胞水平；分析胃癌患者CD4⁺PD-1⁺T细胞与CD4⁺PD-1^-T细胞表型；比较胃癌患者CD4⁺PD-1⁺T细胞与CD4⁺PD-1^-T细胞中的干扰素-γ、肿瘤坏死因子α水平；探讨胃癌患者CD4⁺PD-1⁺T细胞与疾病进展的相关性。 结果观察组CD4⁺PD-1⁺T细胞比例高于对照组（χ²=14.386，P＜0.05）；与CD4⁺PD-1^-T细胞比较，CD4⁺PD-1⁺T细胞中CD45RA⁺CD27⁺、CD45RA⁺CD27^-比例下降（P＜0.05），CD45RA^-CD27⁺比例增加（P＜0.05）；CD4⁺PD-1⁺T细胞表达的干扰素-γ、肿瘤坏死因子α水平低于CD4⁺PD-1^-T细胞（P＜0.05）；CD4⁺PD-1⁺T细胞水平在不同性别、年龄间差异无统计学意义（P＞0.05），晚期胃癌患者CD4⁺PD-1⁺T细胞比例高于早期患者（P＜0.05）。 结论CD4⁺PD-1⁺T细胞在胃癌患者外周血中的表型、功能改变较为明显，且与疾病的进展密切相关，与年龄、性别无关。

Phenotype,function and correlation with clinical stage of CD4+ PD-1+ T cells in peripheral blood of patients with gastric cancer

ObjectiveTo investigate the phenotype, function and correlation with clinical stage of CD4⁺PD-1⁺T cells (programmed cell death receptor 1) in peripheral blood of patients with gastric cancer. Methods70 patients with gastric cancer treated in Changshu Medical Laboratory from January 2019 to December 2020 were selected as the observation group, and 50 healthy people were selected as the control group. The levels of CD4⁺PD-1⁺T cells in the two groups were compared. The phenotypes of CD4⁺PD-1⁺T cells and CD4⁺PD-1^-T cells in patients with gastric cancer were analyzed. The levels of interferon-γ and tumor necrosis factor-αin CD4⁺PD-1⁺T cells were compared. The correlation between CD4⁺PD-1⁺T cells and disease progression in gastric cancer patients were investigated. ResultsThe proportion of CD4⁺PD-1⁺ cells in the observation group was higher than that in the control group (χ²=14.386, P＜0.05); compared with CD4⁺PD-1^-T cells, the ratio of CD45RA⁺CD27⁺ and CD45RA⁺CD27^- cells in CD4⁺PD-1⁺T cells was decreased (P＜0.05), and the ratio of CD45RA^-CD27⁺ cells was increased (P＜0.05); the levels of interferon-γ and tumor necrosis factor-α expressed by CD4⁺PD-1⁺ T cells were lower than those in CD4⁺PD-1^-T cells (P＜0.05); the levels of CD4⁺PD-1⁺T cells were not significantly different between genders and ages (P＞0.05). The proportion of CD4⁺PD-1⁺T cells in patients with advanced gastric cancer was higher than that in patients with early stage (P＜0.05). ConclusionThe phenotype and function of CD4⁺PD-1⁺T cells in peripheral blood of patients with gastric cancer are significantly changed, and are closely related to the progress of the disease, regardless with age or gender.