| 应用组织芯片技术探讨"恶性组织细胞增生症"肿瘤细胞属性及其与EB病毒感染的关系 |
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| 作者姓名： | Lu C Liu W Guo J Li G Li F |
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| 作者单位： | 1. 上海市东方医院
2. 610041,成都,四川大学华西医院病理科 |
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| 基金项目： | 国家自然科学基金资助项目 ( 39770 2 98),四川省科技厅重点课题资助项目 ( 0 1SG0 13 10 3) |
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| 摘 要： | 目的：初步探讨所谓“恶性组织细胞增生症”（简称恶组）肿瘤细胞的属性及其与EB病毒感染之间的关系。方法：用组织芯片技术将5例“恶组”尸检病例的每例不同组织各集成在一张组织芯片上,用免疫组织化学标记链霉素卵白素生物素（LSAB）法检测瘤细胞的免疫表型,用EBER1／2原位杂交检测Epstein-Barr(EB)病毒感染的情况。结果：（1）5例瘤细胞均表达CD45RO、CD3ε、T细胞限制性细胞内抗原－1（TIA－1）、Granzyme B,无瘤细胞表达CD56、CD30、CD20、CD68。（2）5例EBER1／2原位杂交均为阳性（簇型3例、弥漫型2例）。结论：至少部分“恶组”为EB病毒相关的侵袭性T细胞淋巴瘤。
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| 关 键 词： | 组织芯片技术 恶性组织细胞增生症 肿瘤细胞属性 EB病毒感染 |
| 修稿时间： | 2001年10月12 |
Primitive study of the cell lineage and Epstein-Barr virus infection in so called malignant histiocytosis with tissue microarray technique |
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| Lu C,Liu W,Guo J,Li G,Li F.Primitive study of the cell lineage and Epstein-Barr virus infection in so called malignant histiocytosis with tissue microarray technique[J].Chinese Journal of Pathology,2002,31(6):497-501. |
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| Authors: | Lu Changli Liu Weiping Guo Jia Li Gandi Li Fengyuan |
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| Institution: | Department of Pathology, West China Hospital, Sichuan University, Chengdu 610041, China. |
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| Abstract: | OBJECTIVE: To investigate the cell lineage and Epstein-Barr virus infection in previously diagnosed cases of malignant histiocytosis (MH) with tissue microarray technique. METHODS: Using tissue-chips, immunohistochemical staining, in situ hybridization and PCR to analyze 5 autopsy cases of MH. RESULTS: (1) In all 5 cases, positive reactions of CD45RO, CD3 epsilon, TIA-1, Granzyme B were detected in the neoplastic cells, whereas negative reactions were found with CD30, CD20, CD56. (2) All cases revealed EBER1/2 positivity in neoplastic cells. CONCLUSION: Previously diagnosed malignant histiocytosis is an EBV-associated aggressive NK/T cell lymphoma. |
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| Keywords: | Histiocytosis malignant Lymphoma T-cell Epstein-Barr virus infections |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ |
