C57BL/6J小鼠冻融胚胎移植后所得小鼠及其子代生物学特性数据测定与分析

目的探讨C57BL/6J(B6)小鼠冷冻复苏胚胎移植后所得小鼠及其子代的平均体增长和血液生化等部分生物学特性数据的变化。方法实验分为3组。实验组Ⅰ:冻融胚移植组,B6小鼠体外受精后的2-cell胚胎采用EFS法冷冻,复苏后移植到受体(ICR小鼠)输卵管内所产的小鼠(雄鼠30只,雌鼠20只);实验组Ⅱ:冻融胚胎移植所产的小鼠成熟后经自然交配得到的子一代(雄鼠26只,雌鼠17只);对照组:正常饲养和自然交配所得的子代(雌雄小鼠各20只)。3组小鼠饲养至16周龄,每周定时测定体质量,至16周龄时采血,测定各组小鼠血清生化值,并进行分析比较。结果实验组Ⅰ雌雄小鼠初生重和1~16周的平均体重均显著高于对照组(P0.01),实验组Ⅱ雌鼠体重从12周开始均显著高于对照组(P0.01),而雄鼠只有部分周龄与对照组有差异,其余周龄则差异无显著性;实验组Ⅰ和实验组Ⅱ小鼠的血清生化值与对照组相比除AST、TP和Ca等项目无变化外,其余项目均发生了显著或极显著的上调或下调。结论冷冻对胚胎移植所得小鼠及其自然繁殖子一代的平均体增长和血清生化值有一定影响。

Mensurement and analysis of biological characteristics of C57BL/6J mice and their offsprings after frozen-thawed embryo transfer of C57BL/6J mice

Objective To investigate the changes of average body weight gain and serum biochemical indexes of C57BL/6J mice (B6 mouse) and their offspings after frozen-thawed embryo transfer of B6 mice. Methods The mice were divided into three groups in this study. In the experimental group I (E-I, 30 males and 20 females), 2-cell embryos after in-vitro fertilization were collected, and cryopreserved by EFS method, then obtained the offsprings after transplantation of the recovered embryos to oviduct of recipient mice (ICR mouse). In the experimental group II (E-II, 26 males and 17 females), when the mice from E-I grew to maturity, the offsprings were obtained from natural mating of mice from E-I. In the control group (20 males and 20 females), the offsprings came from conventional feeding and natural mating. The three groups of mice were raised to 16 weeks old, weighing the body weight at a regular time intervals, and the serum biochemical indexes were obtained from 16-week-old mice. Then the changes of average body weight and serum biochemical indexes of the mice were analyzed. Results The average body weight of E-I mice was significantly higher than that of control group at each week-age (P<0.01). The average body weight of E-II female mice was significantly higher than that of the control group in 12-16-week old mice (P<0.01), but the average body weight of E-II male mice showed no significant differences compared with the control group except for few weeks. The serum biochemical indexes of E-I and E-II mice were changed in all items except for AST, TP and Ca. Conclusions There are some effects on the average body weight gain and serum biochemical indexes of C57BL/6J mice and their offspings after frozen-thawed embryo transfer.