| 动物笼具和垫料蒸汽灭菌条件的优化 |
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| 引用本文： | 陈霆,张丽芳,金迎春,邓巍,丛喆.动物笼具和垫料蒸汽灭菌条件的优化[J].中国比较医学杂志,2017,27(3):81-83. |
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| 作者姓名： | 陈霆 张丽芳 金迎春 邓巍 丛喆 |
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| 作者单位： | 中国医学科学院, 北京协和医学院, 医学实验动物研究所, 卫生部人类疾病比较医学重点实验室, 国家中医药管理局人类疾病动物模型三级实验室, 北京 100021,中国医学科学院, 北京协和医学院, 医学实验动物研究所, 卫生部人类疾病比较医学重点实验室, 国家中医药管理局人类疾病动物模型三级实验室, 北京 100021,中国医学科学院, 北京协和医学院, 医学实验动物研究所, 卫生部人类疾病比较医学重点实验室, 国家中医药管理局人类疾病动物模型三级实验室, 北京 100021,中国医学科学院, 北京协和医学院, 医学实验动物研究所, 卫生部人类疾病比较医学重点实验室, 国家中医药管理局人类疾病动物模型三级实验室, 北京 100021,中国医学科学院, 北京协和医学院, 医学实验动物研究所, 卫生部人类疾病比较医学重点实验室, 国家中医药管理局人类疾病动物模型三级实验室, 北京 100021 |
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| 基金项目： | 十二五科技重大专项（2012ZX10001001-001，2012ZX10001001-002）；卫生行业科研专项（课题编号：201302006） |
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| 摘 要： | 目的评估几种动物笼具和动物垫料的高压灭菌效果,为安全操作提供依据。方法分别用三种方法高压动物笼具和垫料,用压力蒸汽灭菌化学指示卡和嗜热脂肪芽孢杆菌(ATCC 7953)3MTM压力蒸汽灭菌生物指示剂做为指示高压灭菌效果的指标。结果整包垫料高压,垫料干燥效果不好;直接分装到笼具中高压,灭菌效果不稳定;垫料整包高压后,分装到笼具中再次高压,灭菌效果好,垫料干燥。结论单独高压整包垫料,之后将垫料按实际使用量铺放在笼具里进行再次灭菌的方法效果最好,且垫料干燥。
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| 关 键 词： | 屏障设施 高压灭菌 垫料 笼具 |
| 修稿时间： | 2016/7/28 0:00:00 |
Optimization of steam sterilization conditions for animal cages and beddings |
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| CHEN Ting,ZHANG Li-fang,JIN Ying-chun,DENG Wei and CONG Zhe.Optimization of steam sterilization conditions for animal cages and beddings[J].Chinese Journal of Comparative Medicine,2017,27(3):81-83. |
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| Authors: | CHEN Ting ZHANG Li-fang JIN Ying-chun DENG Wei and CONG Zhe |
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| Institution: | Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health;Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences;Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine;Peking Union Medicine College, Beijing 100021, China,Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health;Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences;Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine;Peking Union Medicine College, Beijing 100021, China,Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health;Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences;Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine;Peking Union Medicine College, Beijing 100021, China,Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health;Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences;Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine;Peking Union Medicine College, Beijing 100021, China and Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health;Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences;Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine;Peking Union Medicine College, Beijing 100021, China |
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| Abstract: | Objective To evaluate the autoclave sterilization effect on several kinds of animal cages and animal bedding, and provide the basis for safe sterilization operation. Methods Beddings and cages were sterilized with three different methods separately, and chemical indicator cards and biological indicator (Bacillus steatothermophilus) in (ATCC-7953) 3MTM pressure-steam sterilization were used to indicate the sterilization effects. Results The beddings were not completely dried when it was sterilized in whole package. The sterilization quality was unstable while the beddings were autoclaved after divided into separate cages. The best sterilization effect and dry beddings were obtained if the whole package was autoclaved at first and then divided into separate cages and sterilized again. Conclusions The results of this study demonstrate that dry beddings and best sterilization effect can be achieved by whole package autoclaved at first followed by sterilization of the bedding material divided into separate cages. |
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| Keywords: | Barrier facility Autoclave Bedding material Cages |
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